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Anti-BRAF / B-Raf Antibody (clone 1H12) IHC-plus™ LS-B3862

Note: This antibody replaces LS-C108748
Catalog Size Price
LS-B3862-50 50 µl Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 1H12] (IgG1) to Human BRAF / B-Raf
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human BRAF / B-Raf
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [1H12]
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Paraffin (1:200 - 1:300)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000)

Specificity and Use

BRAF / B-Raf antibody was raised against purified recombinant fragment of human BRAF (aa 285-401) expressed in E. coli
Human BRAF
Immunohistochemistry: LS-B3861 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3861 was determined to be 1:200.

Packaging

Ascites fluid, 0.03% sodium azide. Sourced in Ascites.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About BRAF / B-Raf

P15056 NM_004333 NP_004324.2

BRAF Antibody, 94 kDa B-raf protein Antibody, B-raf Antibody, BRAF1 Antibody, B Raf Antibody, Proto-oncogene B-Raf Antibody, p94 Antibody, B-RAF1 Antibody, NS7 Antibody, ONCOGENE BRAF Antibody, RAFB1 Antibody

BRAF / B-Raf is a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance.

Immunohistochemistry

Immunohistochemistry
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3862 dilution 1:200.

Immunohistochemistry

Immunohistochemistry
Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3862 dilution 1:200.

Immunohistochemistry

Immunohistochemistry
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3862 dilution 1:200.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of MCF-7 (left) and HepG2 (right) cells using BRAF mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye

Western blot

Western blot
Western blot using BRAF mouse monoclonal antibody against HeLa (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.

Requested From: 
Date Requested: 12/11/2017

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