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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B3862-50 50 µl $425 
BRAF / B-Raf Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
BRAF / B-Raf Antibody - Human Breast, Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)
BRAF / B-Raf Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
BRAF / B-Raf Antibody - Confocal immunofluorescence of MCF-7 (left) and HepG2 (right) cells using BRAF mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye
BRAF / B-Raf Antibody - Western blot using BRAF mouse monoclonal antibody against HeLa (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
BRAF / B-Raf Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
BRAF / B-Raf Antibody - Human Breast, Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)
BRAF / B-Raf Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
BRAF / B-Raf Antibody - Confocal immunofluorescence of MCF-7 (left) and HepG2 (right) cells using BRAF mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye
BRAF / B-Raf Antibody - Western blot using BRAF mouse monoclonal antibody against HeLa (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
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IHCPlus™ BRAF / B‑Raf Antibody Monoclonal ELISA,IF,IHC,WB LS‑B3862

IHCPlus™ BRAF / B‑Raf Antibody Monoclonal ELISA,IF,IHC,WB LS‑B3862

Note: This antibody replaces LS-C108748, LS-C758759, LS-C355318
Antibody:
Mouse Monoclonal to Human BRAF / B-Raf
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3862-50
50 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human BRAF / B-Raf
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
B-Raf antibody LS-B3862 is an unconjugated mouse monoclonal antibody to human B-Raf (BRAF ). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BRAF / B-Raf
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
1H12
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC - Paraffin (1:200 - 1:400)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified recombinant fragment of human BRAF expressed in E. Coli encoding aa 285-401.
Specificity
Human, Mouse
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BRAF / B-Raf
P15056 NM_004333 NP_004324.2

Validation

BRAF / B-Raf Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)

BRAF / B-Raf Antibody - Human Breast, Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast, Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast, Carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)

BRAF / B-Raf Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

BRAF / B-Raf Antibody - Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

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Requested From: United States
Date Requested: 12/10/2019