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IHCPlus™ BPOZ / ABTB1 Antibody aa465‑478 Polyclonal IHC,Peptide‑ELISA,WB LS‑B3228

Catalog Number Size Price
LS-B3228-50 50 µg (0.5 mg/ml) $425 
Anti-ABTB1 / BPOZ antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-ABTB1 / BPOZ antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Antibody staining (1 ug/ml) of Human Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Anti-ABTB1 / BPOZ antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-ABTB1 / BPOZ antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Antibody staining (1 ug/ml) of Human Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
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IHCPlus™ BPOZ / ABTB1 Antibody aa465‑478 Polyclonal IHC,Peptide‑ELISA,WB LS‑B3228

Note: This antibody replaces LS-C54589, LS-C77157, LS-C45533
Antibody:
Goat Polyclonal to Human BPOZ / ABTB1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Dog, Hamster, Horse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3228-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human BPOZ / ABTB1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Dog, Hamster, Horse
Format:
Unconjugated, Unmodified

Specifications

Description
ABTB1 antibody LS-B3228 is an unconjugated goat polyclonal antibody to ABTB1 (BPOZ ) from human, mouse, dog and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BPOZ / ABTB1
Host
Goat
Reactivity
Human, Monkey, Mouse, Dog, Hamster, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence RALEDLLVSIGLDC, from the C Terminus of the protein sequence according to NP_115937.1NP_742024.1NP_742025.1.
Blocking Peptide
LS-E25253 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3228. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa465-478
Specificity
Human ABTB1 / BPOZ. This antibody is expected to recognise all reported isoforms (NP_115937.1; NP_742024.1; NP_742025.1).
Usage
Immunohistochemistry: LS-B3228 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3228 was determined to be 3.75 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BPOZ / ABTB1
Q969K4 NM_032548 NP_115937.1

Validation

Anti-ABTB1 / BPOZ antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
IHC Validated
Anti-ABTB1 / BPOZ antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-ABTB1 / BPOZ antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.

Anti-ABTB1 / BPOZ antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
IHC Validated
Anti-ABTB1 / BPOZ antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-ABTB1 / BPOZ antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.

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Requested From: United States
Date Requested: 9/22/2019