Clostridium botulinum Botulinum Neurotoxin Type A

Mouse

Clostridium botulinum (tested or 100% immunogen sequence identity)

IgG2b,k Monoclonal

Unconjugated

Tissue culture supernatant

Unmodified

Synthetic peptide analog of the C-Terminus of BoTox-A.

C-Terminus

Recognizes BoTox-A (1280-1292), and BoTox-A, but does not react with BoTox-B, BoTox-C, BoTox-E, or BoTox-F (1272-1280). Binds to intact whole BoTox-A by ELISA, RIA and cell staining techniques. Found to inhibit the binding of BoTox-A to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin, and prior incubation of the antibody with the synthetic peptide immunogen has been shown to eliminate the inhibition of cellular binding by the antibody.

• Immunofluorescence
• ELISA
• Functional Assay
• Radioimmunoassay

Suitable for use in ELISA, RIA, Immunofluorescence and Inhibition of BoTox-A binding to Neuro2A Cells. The staining pattern was observed using epifluorescent microscopy. Inhibition: Inhibits the binding of BoTox-A to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin, and prior incubation of the antibody with the synthetic peptide. The immunogen has been shown to eliminate the inhibition of cellular binding by the antibody.

Supplied as a liquid.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10845 NCBI: P10845