Clostridium botulinum Botulinum Neurotoxin Type A
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- ICC (1:25)
- Functional Assay
Synthetic peptide analogue of the carboxyl terminal region of C. botulinum Toxin A.
Recognizes and binds to intact whole C. botulinum Toxin A, amino acids 1280-1292. Has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of C. botulinum Toxin A. Inhibits the entry of C. botulinum Toxin A to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin. Cross-reactivity: C. botulinum Toxin A (1280-1292): 100%, C. botulinum Toxin A: 100%C. botulinum Toxin B, C, E and F (1272-1280): 0%.
Suitable for use in ELISA, Immunofluorescence, Indirect Immunocytochemistry and RIA. Immunocytochemistry (Indirect): 1:12.5. Note: Prior incubation of the antibody with synthetic peptide immunogen has been shown to eliminate the inhibition of cellular binding by the antibody.
PBS, 40% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.