Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Western blot (0.1 - 0.2 µg/ml)
ELISA (0.5 - 1 µg/ml)
Specificity and Use
BMP2 + BMP4 antibody was raised against e. coli-derived rhBMP-2.
BMP-2, BMP-4 Immunogen: E. coli-derived rhBMP-2.
Western blot-This antibody can be used at 0.1-0.2 µg/ml with the appropriate secondary reagents to detect human BMP-2 and human BMP-4. The detection limit for rhBMP-2 and rhBMP-4 is approximately 20 ng/lane under non-reducing and reducing conditions. Immunohistochemistry-This antibody can be used with the appropriate secondary reagents to detect human BMP-2/BMP-4. An experimental protocol is listed below. Cells (cultured or recently collected) may be fixed for 20 minutes at room temperature with freshly prepared 1-2% paraformaldehyde/PBS (pH 7.4). Three to five washes of cells in PBS (15 minutes each) is usually required after fixation and before application of primary antibodies. Labeling may be obtained by incubating cells overnight at 4° C with 2-5 µg/ml anti-human BMP-2/4 antibodies. Tissues may be dissected from experimental animals that were fixed by vascular perfusion with 4% paraformaldehyde/PBS (pH 7.4) and followed by perfusion with a 10% sucrose solution in 0.1 M phosphate buffer (pH 7.2). Adequate labeling may be achieved on 5-15 micron thick cryostat sections by incubating them with primary antibodies diluted to 5-15 µg/ml. On free-floating sections, primary antibodies should be diluted to 0.5-2 µg/ml to reduce background staining. Direct ELISA-This antibody can be used at 0.5-1.0 µg/ml with the appropriate secondary reagents to detect human BMP-2 and human BMP-4. The detection limit for rhBMP-2 and rhBMP-4 is approximately 15 ng/well. Detection of labeling on cells and tissues may be done by employing either fluorescence or non-fluorescence enzymatic protocols. Note: Due to accumulation of autofluorescent pigment lipofucsin in primate neuronal tissues, the use of fluorescent probes such as FITC or Cy3 is not recommended. Enzymatic protocols (e. g. DAB, AEC or immunogold-silver staining) may be used instead.