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Anti-BMP2 + BMP4 Antibody LS-C149885

Catalog Size Price
LS-C149885-25 25 µg Unavailable
LS-C149885-100 100 µg Unavailable

Related Products

1 BMP2 + BMP4 Antibody

Most Popular BMP2 + BMP4 Antibodies

Anti-BMP2 + BMP4 Antibody (AP) LS-C86962
Goat Polyclonal to Human BMP2 + BMP4
Human, Rat
AP Conjugated
Anti-BMP2 + BMP4 Antibody LS-C149885
Goat Polyclonal (IgG) to Human BMP2 + BMP4
Human
IHC, Western blot, ELISA
Unconjugated

100% Guaranteed
Goat Polyclonal (IgG) to Human BMP2 + BMP4
Human
IHC, Western blot, ELISA
Unconjugated

Details

Human BMP2 + BMP4
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

BMP2 + BMP4 antibody was raised against e. coli-derived rhBMP-2.
BMP-2, BMP-4 Immunogen: E. coli-derived rhBMP-2.
Western blot-This antibody can be used at 0.1-0.2 µg/ml with the appropriate secondary reagents to detect human BMP-2 and human BMP-4. The detection limit for rhBMP-2 and rhBMP-4 is approximately 20 ng/lane under non-reducing and reducing conditions. Immunohistochemistry-This antibody can be used with the appropriate secondary reagents to detect human BMP-2/BMP-4. An experimental protocol is listed below. Cells (cultured or recently collected) may be fixed for 20 minutes at room temperature with freshly prepared 1-2% paraformaldehyde/PBS (pH 7.4). Three to five washes of cells in PBS (15 minutes each) is usually required after fixation and before application of primary antibodies. Labeling may be obtained by incubating cells overnight at 4° C with 2-5 µg/ml anti-human BMP-2/4 antibodies. Tissues may be dissected from experimental animals that were fixed by vascular perfusion with 4% paraformaldehyde/PBS (pH 7.4) and followed by perfusion with a 10% sucrose solution in 0.1 M phosphate buffer (pH 7.2). Adequate labeling may be achieved on 5-15 micron thick cryostat sections by incubating them with primary antibodies diluted to 5-15 µg/ml. On free-floating sections, primary antibodies should be diluted to 0.5-2 µg/ml to reduce background staining. Direct ELISA-This antibody can be used at 0.5-1.0 µg/ml with the appropriate secondary reagents to detect human BMP-2 and human BMP-4. The detection limit for rhBMP-2 and rhBMP-4 is approximately 15 ng/well. Detection of labeling on cells and tissues may be done by employing either fluorescence or non-fluorescence enzymatic protocols. Note: Due to accumulation of autofluorescent pigment lipofucsin in primate neuronal tissues, the use of fluorescent probes such as FITC or Cy3 is not recommended. Enzymatic protocols (e. g. DAB, AEC or immunogold-silver staining) may be used instead.

Packaging

Lyophilized from PBS, pH 7.4, 5% trehalose
Reconstitute at 0.2 mg/mL in sterile PBS
Store at -20°C.
For research use only.

About BMP2 + BMP4


Requested From: 
Date Requested: 10/18/2017

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