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Anti-BMP2 Antibody IHC-plus™ LS-B7430

Note: This antibody replaces LS-C104476, LS-C43125

Ordering

Wt. Vol. Conc. Price
50 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BMP2 Antibodies

Anti-BMP2 Antibody (aa86-102) IHC-plus™ LS-B785
Rabbit Polyclonal to Human BMP2
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-BMP2 Antibody IHC-plus™ LS-B7430
Rabbit Polyclonal (IgG) to Human BMP2
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human BMP2
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated

Details

Human BMP2
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (1.3 - 1.9 µg/ml)

Specificity and Use

BMP2 antibody was raised against e.coli derived recombinant Human BMP-2.
Human BMP-2
Immunohistochemistry: LS-B7430 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hBMP-2 (200 ng/ml), a concentration of 1.3-1.9 ug/ml of this antibody is required. ELISA: To detect hBMP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BMP-2 (LS-C104469) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBMP-2. Western Blot: To detect hBMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Reconstitute in sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About BMP2

P12643 NM_001200 NP_001191.1

BMP2 Antibody, BDA2 Antibody, BMP-2 Antibody, BMP-2A Antibody, BMP2A Antibody, Bone morphogenetic protein 2 Antibody, Bone morphogenetic protein 2A Antibody

BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia (myositis) ossificans progressiva.

Immunohistochemistry

Immunohistochemistry
Anti-BMP2 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7430 concentration 5 ug/ml.

Western blot

Western blot
Western Blot (non-reducing) of BMP2 antibody LS-B7430

Western blot

Western blot
Western Blot (reducing) of BMP2 antibody LS-B7430

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of BMP2 antibody LS-B7430

Requested From: United States
Date Requested: 12/4/2016

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