Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
ICC (5 - 15 µg/ml)
Western blot (0.1 µg/ml)
Immunoprecipitation (25 µg/ml)
Specificity and Use
BMP1 antibody was raised against mouse myeloma cell line NS0-derived recombinant human BMP-1/PCP Ala121-Gln730 Accession # NP_001 190.
Detects human BMP-1/PCP direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human.
Western Blot, 0.1 µg/ml, Recombinant Human BMP-1/PCP. Immunocytochemistry, 5-15 µg/ml, Immersion fixed U2OS human osteosarcoma cell line. Immunoprecipitation, 25 µg/ml, Conditioned cell culture medium spiked with Recombinant Human BMP-1/PCP.
Lyophilized from 0.2 µm filtered PBS, pH 7.4, 5% trehalose
Sterile PBS. Resuspend to 500 ul
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
BMP1 encodes a Metallopeptidase M12A type protease that is comprised of an amino terminal signal peptide, prodomain and an astacin-like protease domain followed by CUB and EGF-like motifs. BMP1 protein has been shown to cleave chordin, the C-terminal propeptides of procollagen I, II, III and VII, pro-lysyl oxidase, probiglycan, and laminin-5, and its expression has been shown to induce cartilage and bone formation in vivo. At least six spliced variants have been reported.