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Anti-BLZF1 Antibody (aa156-184) LS-C156829

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human BLZF1
Human
Immunofluorescence, Western blot
Unconjugated

Details

Human BLZF1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

LS-E10213 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156829. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa156-184
This BLZF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the Central region of human BLZF1.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About BLZF1

Q9H2G9 NM_003666 NP_003657.1

BLZF1 Antibody, Cytoplasmic protein Antibody, Golgin-45 Antibody, JEM-1 Antibody, JEM-1s Antibody, JEM-1short protein Antibody, JEM1 Antibody

Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.

Immunofluorescence

Immunofluorescence
Fluorescent image of A549 cell stained with BLZF1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BLZF1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). BLZF1 immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot
Western blot of BLZF1 Antibody in HepG2 cell line lysates (35 ug/lane). BLZF1 (arrow) was detected using the purified antibody.

Requested From: 
Date Requested: 4/24/2017

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