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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BLM Antibody (phospho‑Thr99) LS‑C198690
BLM antibody LS-C198690 is an unconjugated rabbit polyclonal antibody to human BLM. Validated for IF, IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C198690-50
50 µl
Unavailable
LS-C198690-100
100 µl
Unavailable

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Product Description

BLM antibody LS-C198690 is an unconjugated rabbit polyclonal antibody to human BLM. Validated for IF, IHC, Peptide-ELISA and WB.
About BLM
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE). P54132 NM_000057 NP_000048.1

BLM Antibody, Bloom syndrome Antibody, Bloom syndrome protein Antibody, BS Antibody, Bloom's syndrome protein Antibody, DNA helicase, RecQ-like type 2 Antibody, RECQ2 Antibody, RECQL2 Antibody, RECQL3 Antibody, RecQ protein-like 3 Antibody


Specifications

Target
Human BLM
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BLM antibody was raised against synthetic peptide derived from human Bloom Syndrome around the phosphorylation site of Thr99.
Epitope
pThr99
Specificity
Bloom Syndrome (Phospho-Thr99) Antibody detects endogenous levels of Bloom Syndrome only when phosphorylated at threonine99.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HeLa cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HeLa cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HepG2 cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HepG2 cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HeLa cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HeLa cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HepG2 cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HepG2 cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HeLa cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HeLa cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HepG2 cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HepG2 cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HeLa cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HeLa cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HepG2 cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HepG2 cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The lane on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/23/2018

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