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Anti-BLK Antibody (aa301-350) LS-C80808

Catalog Size Price
LS-C80808-100 100 µg Unavailable

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57 BLK Antibodies

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Anti-BLK Antibody (aa241-494, FITC) LS-C695215
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Western blot of BLK antibody.
Anti-BLK Antibody (aa241-494, Cy3) LS-C703958
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Western blot of BLK antibody.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human BLK
Human, Monkey, Mouse, Rat, Hamster, Pig
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human BLK
Rabbit
Human, Monkey, Mouse, Rat, Hamster, Pig (tested or 100% immunogen sequence identity)
Bovine, Dog, Guinea pig, Horse, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Protein A purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

BLK antibody was raised against synthetic peptide located between aa301-350 of human BLK (P51451, NP_001706). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Pig (100%); Dog, Bovine, Rabbit, Horse, Guinea pig (92%); Galago, Platypus (85%); Chicken (84%).
LS-E16784 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80808. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa301-350
Human BLK
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 2.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
100 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About BLK

P51451 NM_001715 NP_001706.2

BLK Antibody, Blk kinase Antibody, B lymphocyte kinase Antibody, B lymphoid tyrosine kinase Antibody, MODY11 Antibody, Protein kinase blk Antibody, Protein tyrosine kinase blk Antibody, B lymphoid kinase Antibody, p55-Blk Antibody, Tyrosine-protein kinase Blk Antibody

Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation.

Western blot

HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/23/2018

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