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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BIRC7 / Livin Antibody (aa285‑295) LS‑C20176
Note: This antibody replaces LS-C71762, LS-C16222
Livin antibody LS-C20176 is an unconjugated goat polyclonal antibody to Livin (BIRC7) from human and monkey. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C20176-100
100 µg (0.5 mg/ml)
Unavailable

Popular BIRC7 / Livin Products

Anti-BIRC7 / Livin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B456 concentration 5 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Anti-BIRC7 / Livin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2018 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
IHC of paraffin-embedded liver tissue using anti-BI mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
IHC of Livin in Bouins-fixed, paraffin-embeddedE13.5 mouse embryo (13.5 days post coitum) Bouins using LS-C148274 at 1:2000. The mother was perfused prior to embryo harvesting. A, brain over view. B, brain. C, spinal cord. D, liver and abdominal wall. Hematoxylin-Eos in counterstain.
Species: Human, Monkey, Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: ICC, Western blot, Immunoprecipitation

Product Description

Livin antibody LS-C20176 is an unconjugated goat polyclonal antibody to Livin (BIRC7) from human and monkey. Validated for Peptide-ELISA and WB.
About BIRC7 / Livin
Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. Q96CA5 NM_022161 NP_071444.1

BIRC7 Antibody, KIAP Antibody, Livin inhibitor of apoptosis Antibody, MLIAP Antibody, ML-IAP Antibody, RNF50 Antibody, RING finger protein 50 Antibody, LIVIN Antibody


Specifications

Target
Human BIRC7 / Livin
Host
Goat
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Failed Applications
  • IHC - Paraffin
Immunogen
BIRC7 / Livin antibody was raised against synthetic peptide C-RAPVRSRVRT from the C-terminus of human BIRC7 / Livin (NP_647478.1; NP_071444.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Rabbit (91%); Marmoset, Panda, Dog, Bovine (82%).
Blocking Peptide
LS-E25224 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C20176. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa285-295
Specificity
Human BIRC7 / Livin. This antibody is expected to recognise both reported isoforms (as represented by NP_647478.1 and NP_071444.1)
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Staining (0.5?g/ml) of Daudi (A), Jurkat (B) and K562 (C) lysates (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Staining (0.5?g/ml) of Daudi (A), Jurkat (B) and K562 (C) lysates (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Staining (0.3?g/ml) of MOLT4 lysate (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Staining (0.3?g/ml) of MOLT4 lysate (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Staining (0.3 ug/ml) of MOLT4 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.
Staining (0.3 ug/ml) of MOLT4 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

Western blot

Staining (0.5?g/ml) of Daudi (A), Jurkat (B) and K562 (C) lysates (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Staining (0.5?g/ml) of Daudi (A), Jurkat (B) and K562 (C) lysates (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Staining (0.3?g/ml) of MOLT4 lysate (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Staining (0.3?g/ml) of MOLT4 lysate (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Staining (0.3 ug/ml) of MOLT4 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.
Staining (0.3 ug/ml) of MOLT4 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

Western blot

Staining (0.5?g/ml) of Daudi (A), Jurkat (B) and K562 (C) lysates (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Staining (0.5?g/ml) of Daudi (A), Jurkat (B) and K562 (C) lysates (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Staining (0.3?g/ml) of MOLT4 lysate (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Staining (0.3?g/ml) of MOLT4 lysate (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Staining (0.3 ug/ml) of MOLT4 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.
Staining (0.3 ug/ml) of MOLT4 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

Western blot

Staining (0.5?g/ml) of Daudi (A), Jurkat (B) and K562 (C) lysates (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Staining (0.5?g/ml) of Daudi (A), Jurkat (B) and K562 (C) lysates (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Staining (0.3?g/ml) of MOLT4 lysate (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Staining (0.3?g/ml) of MOLT4 lysate (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Staining (0.3 ug/ml) of MOLT4 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.
Staining (0.3 ug/ml) of MOLT4 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.


Requested From: 
Date Requested: 6/20/2018

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