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Catalog Number Size Price
LS-C154189-1 1 mg (1 mg/ml) $749 
Biotin Antibody - ELISA results of purified Goat anti-Biotin Antibody Peroxidase Conjugated tested against purified biotin. Each well was coated in duplicate with 1.0 µg of Bovine Albumin Biotin Conjugated  The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

Polyclonal Goat Biotin Antibody (HRP, IHC, WB) LS‑C154189

Polyclonal Goat Biotin Antibody (HRP, IHC, WB) LS‑C154189

Antibody:
Biotin Goat Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA, DB, EM
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$749
LS-C154189-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Biotin Goat Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA, DB, EM
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Biotin antibody LS-C154189 is an HRP-conjugated goat polyclonal antibody to Biotin. Validated for DB, ELISA, EM, IHC and WB.
Host
Goat
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, biotinylated bovine serum albumin and Biotinylated IgG.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:3000 - 1:15000)
  • ELISA (1:140000)
  • Dot Blot
  • Electron Microscopy
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024