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Anti-Biotin Antibody LS-C77041

Ordering

Wt. Vol. Conc. Price
- 100 µl - $475
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Biotin Antibodies

Anti-Biotin Antibody (Cy5) LS-C77018
Goat Polyclonal
Immunofluorescence, Flow Cytometry, ELISA
Cy5 Conjugated
Anti-Biotin Antibody (HRP) LS-C77034
Rabbit Polyclonal (IgG)
IHC, Western blot, ELISA
HRP Conjugated
Anti-Biotin Antibody LS-C194408
Mouse Monoclonal (IgG1)
ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2a,k)
Western blot, ELISA
Unconjugated

Details

Biotin
Mouse
IgG2a,k Monoclonal
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Western blot
  • ELISA (1:20000)

Specificity and Use

Biotin antibody was raised against biotinylated denatured ovalbumin.
Specific for and reacts strongly with biotin. Strong reaction is seen in ELISA with biotin/OA directly coated onto the microtiter well, also when tested in sandwich ELISA in combination with streptavidin. In Western blotting after SDS-PAGE reacts with biotinylated proteins in both reduced and non-reduced forms.
Suitable for use in ELISA, Western Blot. ELISA: 1:20000.

Packaging

PBS, pH 7.4, 15 mM sodium azide, 40% glycerol.
For research use only.

About Biotin

Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size means the biological activity of the protein will most likely be unaffected. This process is called biotinylation. Because both streptavidin and avidin bind biotin with high affinity (Kd of 10-14 mol/l to 10-15 mol/l) and specificity, biotinylated proteins of interest can be isolated from a sample by exploiting this highly stable interaction.

Requested From: United States
Date Requested: 12/11/2016

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