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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Biotin Antibody LS‑C77002
Antibody
Biotin antibody LS-C77002 is an unconjugated goat polyclonal antibody to Biotin. Validated for ELISA.
Catalog
Size
Price
LS-C77002-500
500 µg
Unavailable

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Product Description

Biotin antibody LS-C77002 is an unconjugated goat polyclonal antibody to Biotin. Validated for ELISA.
About Biotin
Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size means the biological activity of the protein will most likely be unaffected. This process is called biotinylation. Because both streptavidin and avidin bind biotin with high affinity (Kd of 10-14 mol/l to 10-15 mol/l) and specificity, biotinylated proteins of interest can be isolated from a sample by exploiting this highly stable interaction.

Specifications

Target
Biotin
Host
Goat
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ELISA (1:4000 - 1:20000)
Immunogen
Biotin antibody was raised against biotin conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity
Recognizes biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, biotinylated IgG and biotinylated BSA. No cross-reactivity was observed against anti-pepsin or anti-goat IgG F(c).
Usage
Suitable for use in ELISA and highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, high titer and specificity. ELISA: 1:4000-1:20000 using 1 ug biotinylated BSA.
Presentation
Sterile-filtered PBS, pH 7.2, 0.01% sodium azide, 40% glycerol.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/25/2018

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