Biotin

Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size means the biological activity of the protein will most likely be unaffected. This process is called biotinylation. Because both streptavidin and avidin bind biotin with high affinity (Kd of 10-14 mol/l to 10-15 mol/l) and specificity, biotinylated proteins of interest can be isolated from a sample by exploiting this highly stable interaction. The sample is incubated with streptavidin/avidin beads, allowing capture of the biotinylated protein of interest. Any other proteins binding to the biotinylated molecule will also stay with the bead and all other unbound proteins can be washed away. However, due to the extremely strong streptavidin-biotin interaction, very harsh conditions are needed to elute the biotinylated protein from the beads (typically 6M guanidine HCl at pH 1.5), which often will denature the protein of interest. To circumvent this problem, beads conjugated to monomeric avidin can be used, which has a decreased biotin-binding affinity of ~10-8 mol/l, allowing the biotinylated protein of interest to be eluted with excess free biotin. ELISAs often make use of biotinylated secondary antibodies against the antigen of interest, followed by a detection step using streptavidin conjugated to a reporter molecule, such as horseradish peroxidase or alkaline phosphatase.

Biotin Target Details

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Products

Antibodies (96)

ELISA Kits (1)

Type

Competitive EIA (1)

Primary (96)

Target

Biotin (97)

Reactivity

Human (2)

All species (2)

Goat (5)

Synthetic (1)

Application

IHC (36)

IHC-P (6)

WB (50)

Flo (30)

DB (4)

ELISA (62)

EM (2)

FLISA (6)

ICC (7)

ID (1)

IF (30)

IP (2)

ISH (2)

Micro (2)

PLA (1)

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Host

rabbit (14)

mouse (27)

goat (55)

Isotype

IgG (31)

IgG F(ab')2 (7)

IgG Fab (5)

IgG1 (21)

IgG1,k (1)

IgG2a,l (2)

IgG2b,k (1)

IgG3 (2)

Clonality

monoclonal mc (27)

polyclonal pc (67)

recombinant monoclonal rmc (2)

Clone

15D9 (1)

4C7.D5 (2)

B25 (BGN/09/6225) (1)

Bio-8 (1)

BIO-8 (1)

BTN/2032R (2)

Hyb-8 (2)

SB58c (5)

SPM375 (2)

Format

96-Well Microplate (1)

AP Conjugated (10)

APC Conjugated (1)

Azide-free (4)

Biotin Conjugated (5)

BSA-free (2)

Carrier-free (1)

Concentrated (1)

Cy2 Conjugated (1)

Cy3 Conjugated (1)

Cy3.5 Conjugated (1)

Cy5 Conjugated (1)

Cy5.5 Conjugated (1)

FITC Conjugated (14)

HRP Conjugated (14)

PE Conjugated (4)

Rhodamine Conjugated (2)

RPE Conjugated (3)

TR Conjugated (5)

TRITC Conjugated (5)

Unconjugated (28)

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Publications

No (96)

Yes (1)

Sample Type

Plasma (1)

Serum (1)

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Biotin (Competitive EIA) ELISA Kit - LS-F10160

(4.0) Rating

(1) Publication(s)

Type:

Competitive EIA

Format:

96-Well Microplate

Range:

12.5 - 200 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$870

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Biotin Antibody LS-C154217

Antibody:

Biotin Rabbit Polyclonal Antibody

Applications:

ELISA, IHC, WB

Format:

Unconjugated

Price:

100 µg/$424

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Biotin Antibody LS-C194408

Antibody:

Biotin Mouse Monoclonal Antibody

Applications:

ELISA

Format:

Unconjugated

Price:

1 mg/$362

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Biotin Antibody (clone 4C7.D5) LS-C153773

Antibody:

Biotin Mouse Monoclonal (4C7.D5) Antibody

Applications:

ELISA, IF, WB

Format:

Unconjugated

Price:

100 µg/$400

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Biotin Antibody LS-C154388

Antibody:

Biotin Goat Polyclonal Antibody

Applications:

ELISA, IHC, WB

Format:

Unconjugated

Price:

1 mg/$559

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Biotin Antibody (clone 15D9) LS-C153295

Antibody:

Biotin Mouse Monoclonal (15D9) Antibody

Applications:

ELISA, IHC, WB

Format:

Unconjugated

Price:

50 µg/$440; 100 µg/$473

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Biotin Antibody (FITC) LS-C61569

Antibody:

Biotin Goat Polyclonal (FITC) Antibody

Applications:

DB, FLISA, IF, WB

Format:

FITC Conjugated

Price:

1 mg/$703

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Biotin Antibody (Cy5) LS-C77018

Antibody:

Biotin Goat Polyclonal (Cy5) Antibody

Applications:

ELISA, Flo, IF

Format:

Cy5 Conjugated

Price:

1 mg/$973

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Biotin Antibody LS-C77003

Antibody:

Biotin Goat Polyclonal Antibody

Applications:

ELISA, IP, WB

Format:

Unconjugated

Price:

2 ml/$665

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Biotin Antibody (FITC) LS-C77024

Antibody:

Biotin Goat Polyclonal (FITC) Antibody

Applications:

Flo, IF

Format:

FITC Conjugated

Price:

500 µg/$720; 1 mg/$1,099

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Biotin Antibody (clone 4C7.D5) LS-C209200

Antibody:

Biotin Mouse Monoclonal (4C7.D5) Antibody

Applications:

ELISA, Flo, IF, IHC, WB

Format:

Unconjugated

Price:

1 mg/$2,125

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Biotin Antibody (clone SPM375) LS-C392498

Antibody:

Biotin Mouse Monoclonal (SPM375) Antibody

Applications:

Flo, IF, IHC, IHC-P, WB

Format:

Unconjugated

Price:

20 µg/$371; 100 µg/$574

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Biotin Antibody (clone SPM375, Azide-free, BSA-free) LS-C392495

Antibody:

Biotin Mouse Monoclonal (Azide-free, BSA-free) (SPM375) Antibody

Applications:

Flo, IF, IHC, IHC-P, WB

Format:

Unconjugated, Azide-free, BSA-free

Price:

100 µg/$574

• Unconjugated
• Unconjugated, Azide-free, BSA-free

• Unconjugated
• Unconjugated, Azide-free, BSA-free

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Biotin Antibody (clone Hyb-8) LS-C357731

Antibody:

Biotin Mouse Monoclonal (Hyb-8) Antibody

Applications:

Flo, IF, IHC, IHC-P, WB

Format:

Unconjugated

Price:

20 µg/$371; 100 µg/$574

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Biotin Antibody (clone Hyb-8, Azide-free, BSA-free) LS-C389762

Antibody:

Biotin Mouse Monoclonal (Azide-free, BSA-free) (Hyb-8) Antibody

Applications:

Flo, IF, IHC, IHC-P, WB

Format:

Unconjugated, Azide-free, BSA-free

Price:

100 µg/$574

• Unconjugated
• Unconjugated, Azide-free, BSA-free

• Unconjugated
• Unconjugated, Azide-free, BSA-free

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Biotin Antibody (clone BTN/2032R) LS-C751509

Antibody:

Biotin Rabbit Recombinant Monoclonal (BTN/2032R) Antibody

Reactivity:

All species

Applications:

IHC-P, ISH

Format:

Unconjugated

Price:

20 µg/$371; 100 µg/$574

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Biotin Antibody (clone BTN/2032R, Azide-free, Carrier-free) LS-C751507

Antibody:

Biotin Rabbit Recombinant Monoclonal (Azide-free, Carrier-free) (BTN/2032R) Antibody

Reactivity:

All species

Applications:

IHC-P, ISH

Format:

Unconjugated, Azide-free, Carrier-free

Price:

100 µg/$574

• Unconjugated
• Unconjugated, Azide-free, Carrier-free

• Unconjugated
• Unconjugated, Azide-free, Carrier-free

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Biotin Antibody (clone SB58c, HRP) LS-C351771

Antibody:

Biotin Mouse Monoclonal (HRP) (SB58c) Antibody

Applications:

ELISA

Format:

HRP Conjugated

Price:

1 ml/$374

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Biotin Antibody (RPE) LS-C154202

Antibody:

Biotin Goat Polyclonal (RPE) Antibody

Applications:

ELISA, Flo, IF, IHC, WB

Format:

RPE Conjugated

Price:

1 ml/$749

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Biotin Antibody LS-C835459

Antibody:

Biotin Rabbit Polyclonal Antibody

Applications:

ELISA, ICC, IHC, WB

Format:

Unconjugated

Price:

1 ml/$290

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Biotin Antibody LS-C835463

Antibody:

Biotin Goat Polyclonal Antibody

Applications:

ELISA, ICC, IHC, WB

Format:

Unconjugated

Price:

1 ml/$285

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Biotin Antibody (RPE) LS-C154302

Antibody:

Biotin Rabbit Polyclonal (RPE) Antibody

Applications:

ELISA, Flo, IF, WB

Format:

RPE Conjugated

Price:

100 µl/$502

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Biotin Antibody (HRP) LS-C835464

Antibody:

Biotin Rabbit Polyclonal (HRP) Antibody

Applications:

ELISA, ICC, IHC, WB

Format:

HRP Conjugated

Price:

1 ml/$339

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Biotin Antibody (HRP) LS-C835465

Antibody:

Biotin Goat Polyclonal (HRP) Antibody

Applications:

ELISA, ICC, IHC, WB

Format:

HRP Conjugated

Price:

1 ml/$334

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Biotin Antibody LS-C77001

Antibody:

Biotin Goat Polyclonal Antibody

Applications:

ELISA, ID, IHC, IP, WB

Format:

Unconjugated

Price:

1 mg/$1,099

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