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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BID Antibody (C‑Terminus) IHC‑plus™ LS‑B2715 Best Seller
Note: This antibody replaces LS-C444, LS-C57095, LS-C43113
BID antibody LS-B2715 is an unconjugated rabbit polyclonal antibody to BID from human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2715-50
50 µg (1 mg/ml)
Unavailable

Popular BID Products

Anti-BID antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4062 concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Two cores (A and B) from a human ovarian carcinoma formalin-fixed, paraffin-embedded tissue microarray stained for Bid expression using LS-C148235 at 1:2000. Hematoxylin-Eos in counterstain.
Species: Mouse, Human, Rat, Dog, Gerbil
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Anti-BID antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7843 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-BID antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

BID antibody LS-B2715 is an unconjugated rabbit polyclonal antibody to BID from human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About BID
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. P55957 NM_001196 NP_001187.1

BID Antibody, Apoptic death agonist Antibody, BID isoform L(2) Antibody, BID isoform Si6 Antibody, Bh3 Antibody, FP497 Antibody, p22 BID Antibody, BID isoform ES(1b) Antibody, Desmocollin type 4 Antibody, Human BID coding sequence Antibody


Specifications

Target
Human BID
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30079 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B2715. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Usage
Immunohistochemistry: LS-B2715 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2715 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-BID antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.
Anti-BID antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.

Immunohistochemistry

Anti-BID antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.
Anti-BID antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 ug/ml.
Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 µg/mL.
Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 µg/mL.

Western blot

Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.
Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Western blot

Western blot of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Western blot of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunohistochemistry

Anti-BID antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.
Anti-BID antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.

Immunohistochemistry

Anti-BID antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.
Anti-BID antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 ug/ml.
Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 µg/mL.
Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 µg/mL.

Western blot

Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.
Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Western blot

Western blot of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Western blot of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunohistochemistry

Anti-BID antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.
Anti-BID antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.

Immunohistochemistry

Anti-BID antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.
Anti-BID antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 ug/ml.
Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 µg/mL.
Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 µg/mL.

Western blot

Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.
Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Western blot

Western blot of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Western blot of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunohistochemistry

Anti-BID antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.
Anti-BID antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.

Immunohistochemistry

Anti-BID antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.
Anti-BID antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2715 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 ug/ml.
Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 µg/mL.
Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 µg/mL.

Western blot

Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.
Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Western blot

Western blot of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Western blot of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.


Requested From: 
Date Requested: 6/21/2018

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