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Anti-BID Antibody (aa28-50) LS-C148237

Note: This antibody replaces LS-C4162

Ordering

Wt. Vol. Conc. Price
- 50 µl - $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BID Antibodies

Anti-BID Antibody (clone 1H11) IHC-plus™ LS-B4062
Rat Monoclonal [clone 1H11] (IgG1) to Human BID
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-BID Antibody LS-C148235
Rabbit Polyclonal to Mouse BID
Mouse, Human, Rat, Dog, Gerbil
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-BID Antibody (aa230-241) IHC-plus™ LS-B11168
Goat Polyclonal to Human BID
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human BID
Human, Mouse
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated

Details

Human BID
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC - Paraffin (1:1000 - 1:5000)
  • IHC - Frozen
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)

Specificity and Use

BID antibody was raised against a synthetic peptide corresponding to amino acids 28-50 (CSDNSFRRELDALGHELPVLAPQ) of human Bid (also known as Bid, isoform 2), GenBank no. NP_001187.1. Human Bid is a 195 amino acid protein. Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (87%).
aa28-50
Full-length Bid is known to undergo cleavage/truncation (reviewed in Yin, 2006). Bid was initially found to be cleaved and activated by caspase-8 following death receptor activation. The term "Bid" was first used to described the caspase-8 cleaved/truncated C-terminal Bid. Bid can be also cleaved by other proteases such as Granzyme B, calpains and cathepsin. The C-terminal portion is considered to be the active Bid moiety. This active form can translocate from the cytosol to the mitochondria. Therefore, the appearance of Bid in the mitochondria is considered to be an indication of active Bid. However, it should also be noted that Bid has been shown to translocate to the mitochondria without cleavage in some model systems. The proteolytic cleavage of Bid usually occurs in the unstructured loop region between the alpha 2 and alpha 3 helices, which is between amino acids (aa) 41 and 79 of Bid. For example, the caspase-8/3 cleavage site is at 60 (human) and 59 (mouse). It should be noted that after cleavage, the smaller N-terminal portion of Bid is not necessarily separated from the larger C-terminal portion. The exact size of the C-terminal cleavage products depends on the Bid cleavage sites; generally 11-15 kD C-truncated cleavage forms are generated. Shorter C-terminal Bid cleavage products may result from protease cleavage that involves both caspases and other proteases. Full length Bid is quite stable, but cleaved/truncated forms may have a short half-life (less than 1.5 h) and degrade rapidly. Therefore, a time course may be useful when detecting Bid cleavage fragments.

Packaging

Antiserum, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About BID

P55957 NM_001196 NP_001187.1

BID Antibody, Apoptic death agonist Antibody, BID isoform L(2) Antibody, BID isoform Si6 Antibody, Bh3 Antibody, FP497 Antibody, p22 BID Antibody, BID isoform ES(1b) Antibody, Desmocollin type 4 Antibody, Human BID coding sequence Antibody

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed, paraffin-embedded mouse embryos stained for Bid using LS-C148237 at 1:2000. A. Intra uterine embryo at E.95 days post conception. B. 10.5-11dpc embryo isolated from the yolk sac. Hematoxylin-Eos in counterstain.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed, paraffin-embedded human tissue sections stained for Bid using LS-C148237 at 1:2000. A and A1, Adult pancreas. B and B1, Adult mesencephalon (midbrain). A1 and B1 are higher magnifications of A and B, respectively. Hematoxylin-Eosin counterstain.

Western blot

Western blot
Western blot of Bid using LS-C148237 at 1:2000. LS-C148237 detected in vitro translated Bid, but not in vitro translated Bcl-2, Bcl-XL. Bax, or Bad. These results show that LS-C148237 is specific for Bid and does not cross-react with other Bcl-2 family members.
 

Requested From: United States
Date Requested: 12/4/2016

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