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Catalog Number Size Price
LS-B3257-50 50 µg (0.5 mg/ml) $485 
BHMT Antibody - Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BHMT Antibody - Anti-BHMT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BHMT Antibody - BHMT antibody (0.03µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
BHMT Antibody - Antibody (0.03 ug/ml) staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
BHMT Antibody - Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BHMT Antibody - Anti-BHMT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BHMT Antibody - BHMT antibody (0.03µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
BHMT Antibody - Antibody (0.03 ug/ml) staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human BHMT Antibody (aa391‑402, IHC, WB) LS‑B3257

IHC‑plus™ Polyclonal Goat anti‑Human BHMT Antibody (aa391‑402, IHC, WB) LS‑B3257

Note: This antibody replaces LS-C61713, LS-C121122
Antibody:
BHMT Goat anti-Human Polyclonal (aa391-402) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rat, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B3257-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BHMT Goat anti-Human Polyclonal (aa391-402) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rat, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
BHMT antibody LS-B3257 is an unconjugated goat polyclonal antibody to BHMT (aa391-402) from human. It is reactive with human, rat, hamster and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human BHMT
Synonyms
BHMT | BHMT1
Host
Goat
Reactivity
Human, Monkey, Rat, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-EQQLKELFEKQK, from the C-Terminus of protein sequence according to NP_001704.1 .
Epitope
aa391-402
Specificity
Human BHMT.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B3257 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3257 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BHMT
Q93088 NM_001713 NP_001704.2

Validation

BHMT Antibody - Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

BHMT Antibody - Anti-BHMT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BHMT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BHMT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ BHMT Antibody (aa391-402) for IHC, WB/Western LS-B3257 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)

Publications (2)

Sulfur amino acid metabolism in doxorubicin-resistant breast cancer cells. Ryu CS, Kwak HC, Lee KS, Kang KW, Oh SJ, Lee KH, Kim HM, Ma JY, Kim SK. Toxicology and applied pharmacology. 2011 255:94-102.

Plasma homocysteine level and hepatic sulfur amino acid metabolism in mice fed a high-fat diet. Yun KU, Ryu CS, Oh JM, Kim CH, Lee KS, Lee CH, Lee HS, Kim BH, Kim SK. European journal of nutrition. 2013 52:127-34. (WB; Mouse)

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/19/2024