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Anti-BHMT Antibody (aa391-402) IHC-plus™ LS-B3257

Note: This antibody replaces LS-C61713, LS-C121122

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BHMT Antibodies

Anti-BHMT Antibody (aa391-402) IHC-plus™ LS-B3257
Goat Polyclonal to Human BHMT
Human, Monkey, Rat, Hamster
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-BHMT Antibody (clone 8C11H5) IHC-plus™ LS-B4100
Mouse Monoclonal [clone 8C11H5] (IgG1) to Human BHMT
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Goat Polyclonal to Human BHMT
Human, Monkey, Rat, Hamster
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human BHMT
Goat
Human, Monkey, Rat, Hamster (tested or 100% immunogen sequence identity)
Mouse, Bat, Bovine, Dog, Horse, Pig, Chicken (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • ELISA (1:16000)

Specificity and Use

BHMT antibody was raised against synthetic peptide C-EQQLKELFEKQK from the C-terminus of human BHMT (NP_001704.1 ). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Hamster, Panda (100%); Mouse, Dog, Bat, Bovine, Horse, Pig, Opossum, Chicken (92%); Rat, Elephant, Rabbit (83%).
LS-E26409 - Lyophilized - 100 µg - $145.00
aa391-402
Human BHMT.
Immunohistochemistry: LS-B3257 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3257 was determined to be 2.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

Publications (2)

1
Sulfur amino acid metabolism in doxorubicin-resistant breast cancer cells. Ryu CS, Kwak HC, Lee KS, Kang KW, Oh SJ, Lee KH, Kim HM, Ma JY, Kim SK. Toxicology and applied pharmacology. 2011 255:94-102. [PubMed:21703291]
2
Plasma homocysteine level and hepatic sulfur amino acid metabolism in mice fed a high-fat diet. Yun KU, Ryu CS, Oh JM, Kim CH, Lee KS, Lee CH, Lee HS, Kim BH, Kim SK. European journal of nutrition. 2013 52:127-34. (WB; Mouse) [PubMed:22209966]

About BHMT

Q93088 NM_001713 NP_001704.2

BHMT Antibody, BHMT1 Antibody

Betaine-homocysteine methyltransferase is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in BHMT could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.

Immunohistochemistry

Immunohistochemistry
Anti-BHMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3257 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-BHMT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3257 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.03 ug/ml) staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/9/2016

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