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Anti-BHLHB2 / DEC1 Antibody (C-Terminus) IHC-plus™ LS-B2222

Note: This antibody replaces LS-C48998, LS-C64625


Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Alternate Forms of this Antibody

LS-C142758 Biotin Conjugated
LS-C142759 DY650 Conjugated
LS-C142760 DY488 Conjugated
LS-C142762 DY550 Conjugated

Most Popular BHLHB2 / DEC1 Antibodies

Anti-BHLHB2 / DEC1 Antibody (aa249-298) LS-C30309
Rabbit Polyclonal (IgG) to Human BHLHB2 / DEC1
Human, Monkey, Mouse, Rat, Guinea pig, Hamster, Horse, Pig
Western blot
Western blot Image
Anti-BHLHB2 / DEC1 Antibody (C-Terminus) IHC-plus™ LS-B2222
Rabbit Polyclonal to Human BHLHB2 / DEC1
IHC - Paraffin, Western blot, Immunoprecipitation
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human BHLHB2 / DEC1
IHC - Paraffin, Western blot, Immunoprecipitation (applications tested for the base form of this product only)


Human BHLHB2 / DEC1
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:5000 - 1:15000)
  • Immunoprecipitation (1:5000 - 1:15000) & (1 - 4 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

BHLHB2 / DEC1 antibody was raised against this DEC1 antibody maps to a region between residues 350 and the C-terminus (residue 412) of human Differentially Expressed in Chondrocytes NP_003661.1 (GeneID 8553)
Immunohistochemistry: LS-B2222 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2222 was determined to be 5 ug/ml.


Tris-citrate/phosphate buffer, 0.09% sodium azide.
+4°C, avoid freezing
For research use only.

About BHLHB2 / DEC1

O14503 NM_003670 NP_003661.1

BHLHE40 Antibody, BHLHB2 Antibody, HLHB2 Antibody, SHARP2 Antibody, Stra14 Antibody, SHARP-2 Antibody

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2.


Anti-BHLHE40 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2222 concentration 5 ug/ml. This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.

Western blot

Western blot
Detection of Human DEC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 mcg for WB) and 293T (1 mg/IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-DEC1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and for IP at 3 mcg/mg lysate. DEC1 was also immunoprecipitated using 2 other rabbit anti-DEC1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.

Requested From: United States
Date Requested: 10/28/2016

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