Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
BHLHB2 / DEC1 antibody was raised against the epitope recognized by this antibody maps to a region between residues 350 and the C-terminus (residue 412) of human Differentially Expressed in Chondrocytes using the numbering given in entry NP_003661.1 (GeneID 8553)
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2.
Anti-BHLHE40 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2222 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of Human DEC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 mcg for WB) and 293T (1 mg/IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-DEC1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and for IP at 3 mcg/mg lysate. DEC1 was also immunoprecipitated using 2 other rabbit anti-DEC1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). This image was taken for the unconjugated form of this product. Other forms have not been tested.