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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BF1 / FOXG1 Antibody (aa225‑252) LS‑C166225
FOXG1 antibody LS-C166225 is an unconjugated rabbit polyclonal antibody to FOXG1 (BF1) from human and mouse. Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C166225-400
400 µl
Unavailable

Popular BF1 / FOXG1 Products

FOXG1 antibody LS-C31123 was used in IHC to stain formalin-fixed, paraffin-embedded human liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
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Species: Human, Mouse
Applications: Western blot, Flow Cytometry, ELISA
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Species: Human, Mouse
Applications: Western blot, Flow Cytometry, ELISA
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Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA

Product Description

FOXG1 antibody LS-C166225 is an unconjugated rabbit polyclonal antibody to FOXG1 (BF1) from human and mouse. Validated for Flow, IF and WB.
About BF1 / FOXG1
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of the brain and telencephalon. P55316 NM_005249 NP_005240.3

FOXG1 Antibody, BF1 Antibody, BF2 Antibody, Brain factor 1 Antibody, BF-2 Antibody, FKHL1 Antibody, FKHL2 Antibody, Forkhead box G1A Antibody, Forkhead box protein G1 Antibody, Forkhead box protein G1A Antibody, Forkhead-related protein FKHL1 Antibody, Forkhead-related protein FKHL2 Antibody, FOXG1A Antibody, FOXG1B Antibody, FOXG1C Antibody, Forkhead box protein G1B Antibody, Forkhead-like 2 Antibody, Forkhead-like 4 Antibody, HBF2 Antibody, HBF-1 Antibody, HFK1 Antibody, FKH2 Antibody, FKHL3 Antibody, FKHL4 Antibody, Forkhead box G1 Antibody, Forkhead box G1B Antibody, Forkhead box G1C Antibody, Forkhead-like 1 Antibody, Forkhead-related protein FKHL3 Antibody, HBF-3 Antibody, HBF-G2 Antibody, HFK3 Antibody, Transcription factor bf-2 Antibody, Oncogene QIN Antibody, QIN Antibody, BF-1 Antibody, Brain factor 2 Antibody, FHKL3 Antibody, Forkhead box protein G1C Antibody, Forkhead-like 3 Antibody, HBF-2 Antibody, HFK2 Antibody, KHL2 Antibody


Specifications

Target
Human BF1 / FOXG1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Chicken, Xenopus, Drosophila (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Blocking Peptide
LS-E10321 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166225. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa225-252
Specificity
This FOXG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-252 amino acids from the Central region of human FOXG1.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Fluorescent confocal image of A549 cell stained with FOXG1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.
Fluorescent confocal image of A549 cell stained with FOXG1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot of FOXG1 Antibody in mouse brain tissue lysates (35 ug/lane). FOXG1 (arrow) was detected using the purified antibody.
Western blot of FOXG1 Antibody in mouse brain tissue lysates (35 ug/lane). FOXG1 (arrow) was detected using the purified antibody.

Flow Cytometry

FOXG1 Antibody flow cytometry of U251 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FOXG1 Antibody flow cytometry of U251 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence

Fluorescent confocal image of A549 cell stained with FOXG1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.
Fluorescent confocal image of A549 cell stained with FOXG1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot of FOXG1 Antibody in mouse brain tissue lysates (35 ug/lane). FOXG1 (arrow) was detected using the purified antibody.
Western blot of FOXG1 Antibody in mouse brain tissue lysates (35 ug/lane). FOXG1 (arrow) was detected using the purified antibody.

Flow Cytometry

FOXG1 Antibody flow cytometry of U251 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FOXG1 Antibody flow cytometry of U251 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence

Fluorescent confocal image of A549 cell stained with FOXG1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.
Fluorescent confocal image of A549 cell stained with FOXG1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot of FOXG1 Antibody in mouse brain tissue lysates (35 ug/lane). FOXG1 (arrow) was detected using the purified antibody.
Western blot of FOXG1 Antibody in mouse brain tissue lysates (35 ug/lane). FOXG1 (arrow) was detected using the purified antibody.

Flow Cytometry

FOXG1 Antibody flow cytometry of U251 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FOXG1 Antibody flow cytometry of U251 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence

Fluorescent confocal image of A549 cell stained with FOXG1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.
Fluorescent confocal image of A549 cell stained with FOXG1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot of FOXG1 Antibody in mouse brain tissue lysates (35 ug/lane). FOXG1 (arrow) was detected using the purified antibody.
Western blot of FOXG1 Antibody in mouse brain tissue lysates (35 ug/lane). FOXG1 (arrow) was detected using the purified antibody.

Flow Cytometry

FOXG1 Antibody flow cytometry of U251 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FOXG1 Antibody flow cytometry of U251 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/20/2018

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