Target
Human Betaglycan / TGFBR3
Synonyms
TGFBR3 | Betaglycan | BGCAN | TGF-beta receptor type 3 | TGF-beta receptor type III | TGFR-3 | Betaglycan proteoglycan | Tgf-beta receptors type iii
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-TKSIRDDIPSTQGN, from the internal region of the protein sequence according to NP_003234.2.
Specificity
Human TGFBR3.
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 38kD band in Human Placenta, Lymph Node, and Tonsil lysates and in lysates of cell lines Daudi, Jurkat and MOLT4 after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 93.5kD according to NP_003234.2. The 38kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Betaglycan / TGFBR3