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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Beta Galactosidase Antibody LS‑C153974
Beta Galactosidase antibody LS-C153974 is an unconjugated rabbit polyclonal antibody to e. coli Beta Galactosidase. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C153974-2000
2000 µl
Unavailable

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Applications: IHC, IHC - Paraffin, Western blot

Product Description

Beta Galactosidase antibody LS-C153974 is an unconjugated rabbit polyclonal antibody to e. coli Beta Galactosidase. Validated for ELISA, IHC and WB.

Specifications

Target
E. coli Beta Galactosidase
Host
Rabbit
Reactivity
E. coli (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • IHC (1:500)
  • Western blot (1:500 - 1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Beta Galactosidase (E. coli).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E. coli]. Cross reactivity against Beta Galactosidase from other sources may occur but have not been specifically determined.
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
Reconstitution
2,000 µl deionized water
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Anti-B-Galactosidase Antibody - Western Blot. Western blotting using Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kD (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blot. The membrane was probed with fluorescein conjugated anti-b-Galactosidase ( diluted to 1:10000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results.
Anti-B-Galactosidase Antibody - Western Blot. Western blotting using Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kD (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blot. The membrane was probed with fluorescein conjugated anti-b-Galactosidase ( diluted to 1:10000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results.

Western blot

Anti-B-Galactosidase Antibody - Western Blot. Western blotting using Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kD (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blot. The membrane was probed with fluorescein conjugated anti-b-Galactosidase ( diluted to 1:10000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results.
Anti-B-Galactosidase Antibody - Western Blot. Western blotting using Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kD (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blot. The membrane was probed with fluorescein conjugated anti-b-Galactosidase ( diluted to 1:10000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results.

Western blot

Anti-B-Galactosidase Antibody - Western Blot. Western blotting using Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kD (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blot. The membrane was probed with fluorescein conjugated anti-b-Galactosidase ( diluted to 1:10000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results.
Anti-B-Galactosidase Antibody - Western Blot. Western blotting using Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kD (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blot. The membrane was probed with fluorescein conjugated anti-b-Galactosidase ( diluted to 1:10000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results.

Western blot

Anti-B-Galactosidase Antibody - Western Blot. Western blotting using Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kD (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blot. The membrane was probed with fluorescein conjugated anti-b-Galactosidase ( diluted to 1:10000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results.
Anti-B-Galactosidase Antibody - Western Blot. Western blotting using Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kD (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blot. The membrane was probed with fluorescein conjugated anti-b-Galactosidase ( diluted to 1:10000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results.


Requested From: 
Date Requested: 6/20/2018

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