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Anti-Beta Galactosidase Antibody LS-C153974


Wt. Vol. Conc. Price
- 2000 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular Beta Galactosidase Antibodies

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Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to E. coli Beta Galactosidase
E. coli
IHC, Western blot, ELISA


E. coli Beta Galactosidase
E. coli (tested or 100% immunogen sequence identity)
Delipidated and defibrinated


  • IHC (1:500)
  • Western blot (1:500 - 1:2000)

Specificity and Use

Beta Galactosidase (E. coli).
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E. coli]. Cross reactivity against Beta Galactosidase from other sources may occur but have not been specifically determined.
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
2,000 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About Beta Galactosidase

Western blot

Western blot
Anti-B-Galactosidase Antibody - Western Blot. Western blotting using Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kD (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blot. The membrane was probed with fluorescein conjugated anti-b-Galactosidase ( diluted to 1:10000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 10/27/2016

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