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Anti-Beta Galactosidase Antibody (Biotin) LS-C154062

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Beta Galactosidase Antibodies

Anti-Beta Galactosidase Antibody LS-C63430
Mouse Monoclonal (IgG1) to E. coli Beta Galactosidase
E. coli
ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-Beta Galactosidase Antibody LS-C149093
Chicken Polyclonal to E. coli Beta Galactosidase
E. coli
IHC, ICC
Unconjugated
Anti-Beta Galactosidase Antibody (aa166-677) IHC-plus™ LS-B10038
Rabbit Polyclonal (IgG) to Human Beta Galactosidase
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to E. coli Beta Galactosidase
E. coli
IHC, Western blot, ELISA
Biotin Conjugated

Details

E. coli Beta Galactosidase
Rabbit
E. coli (tested or 100% immunogen sequence identity)
IgG Polyclonal
Biotin
Ion exchange chromatography
Unmodified

Applications

  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA

Specificity and Use

Beta Galactosidase (E. coli).
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified beta-Galactosidase [E. coli]. Cross reactivity against beta-Galactosidase from other sources may occur but have not been specifically determined.
Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus. A 1:1500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 micron) to produce free-floating sections. A 1:5000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy. A 1:5000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. Although a wide range of conditions was reported to be effective, a 1:10000 dilution was noted to show no background and to be suitable for double labeling experiments. Optimal titers for other applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% sodium azide
deionized water
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
For research use only.

About Beta Galactosidase


Requested From: United States
Date Requested: 12/10/2016

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