Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Beclin 1 is a coiled coil protein that interacts with prototypic cellular antiapoptotic gene Bcl2. Beclin 1 is widely expressed with highest levels reported in skeletal muscle. Three alternativaly spliced isoforms have been identified. Allelic deletion of beclin 1 results in a wide variety of cancers including breast cancer.
IHC of paraffin-embedded Human liver tissue using anti-BECN1 mouse monoclonal antibody.
Anti-BECN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody.
Flow cytometry of Jurkat cells, using anti-BECN1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-BECN1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BECN1 antibody, and then analyzed by flow cytometry.