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Catalog Number Size Price
LS-C172820-100 100 µl (1 mg/ml) $434 
BECN1 / Beclin-1 Antibody - IHC of paraffin-embedded Human liver tissue using anti-BECN1 mouse monoclonal antibody.
BECN1 / Beclin-1 Antibody - Anti-BECN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1.
BECN1 / Beclin-1 Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody.
BECN1 / Beclin-1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
BECN1 / Beclin-1 Antibody - Flow cytometry of Jurkat cells, using anti-BECN1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
BECN1 / Beclin-1 Antibody - Flow cytometry of HeLa cells, using anti-BECN1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
BECN1 / Beclin-1 Antibody - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BECN1 antibody, and then analyzed by flow cytometry.
BECN1 / Beclin-1 Antibody - IHC of paraffin-embedded Human liver tissue using anti-BECN1 mouse monoclonal antibody.
BECN1 / Beclin-1 Antibody - Anti-BECN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1.
BECN1 / Beclin-1 Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody.
BECN1 / Beclin-1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
BECN1 / Beclin-1 Antibody - Flow cytometry of Jurkat cells, using anti-BECN1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
BECN1 / Beclin-1 Antibody - Flow cytometry of HeLa cells, using anti-BECN1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
BECN1 / Beclin-1 Antibody - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BECN1 antibody, and then analyzed by flow cytometry.
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Monoclonal Mouse anti‑Human BECN1 / Beclin‑1 Antibody (clone OTI1F1, IHC, IF, WB) LS‑C172820

Monoclonal Mouse anti‑Human BECN1 / Beclin‑1 Antibody (clone OTI1F1, IHC, IF, WB) LS‑C172820

Antibody:
BECN1 / Beclin-1 Mouse anti-Human Monoclonal (OTI1F1) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Rat, Dog
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$434
LS-C172820-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BECN1 / Beclin-1 Mouse anti-Human Monoclonal (OTI1F1) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Rat, Dog
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Beclin-1 antibody LS-C172820 is an unconjugated mouse monoclonal antibody to Beclin-1 (BECN1) from human. It is reactive with human, rat and dog. Validated for Flow, IF, IHC and WB. Cited in 2 publications.
Target
Human BECN1 / Beclin-1
Synonyms
BECN1 | ATG6 | Beclin 1 | Beclin 1, autophagy related | Beclin1 | GT197 | Protein GT197 | VPS30 | Beclin-1
Host
Mouse
Reactivity
Human, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
OTI1F1
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified. Also available Carrier-free.
Immunogen
Full length human recombinant protein of human BECN1 (NP_003757) produced in HEK293T cell.
Specificity
Human Beclin 1
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BECN1 / Beclin-1
Q14457 NM_003766 NP_003757.1

LSBio Ratings

BECN1 / Beclin-1 Antibody (clone OTI1F1) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-C172820 has an LSBio Rating of
Publications (4.1)

Publications (2)

The autophagy inhibitor spautin-1, either alone or combined with doxorubicin, decreases cell survival and colony formation in canine appendicular osteosarcoma cells. Courtney R Schott, Latasha Ludwig, Anthony J Mutsaers, Robert A Foster, Geoffrey A Wood. PloS one. 2018 Oct;13:e0206427.

Beclin-1 is a novel predictive biomarker for canine cutaneous and subcutaneous mast cell tumors. Britta J Knight, Geoffrey A Wood, Robert A Foster, Brenda L Coomber. Veterinary pathology. 2022 Jan;;59:46-56.

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/18/2024