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Anti-BECN1 / Beclin-1 Antibody (C-Terminus) IHC-plus™ LS-B3202

Note: This antibody replaces LS-C19536, LS-C127025

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BECN1 / Beclin-1 Antibodies

Anti-BECN1 / Beclin-1 Antibody (C-Terminus) IHC-plus™ LS-B3202
Rabbit Polyclonal to Human BECN1 / Beclin-1
Human, Mouse
IHC - Paraffin, ICC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-BECN1 / Beclin-1 Antibody (N-Terminus) IHC-plus™ LS-B3203
Rabbit Polyclonal to Human BECN1 / Beclin-1
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-BECN1 / Beclin-1 Antibody (clone 1F1) LS-C172820
Mouse Monoclonal [clone 1F1] (IgG2a) to Human BECN1 / Beclin-1
Human, Rat, Dog
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human BECN1 / Beclin-1
Human, Mouse
IHC - Paraffin, ICC, Western blot, ELISA
Unconjugated

Details

Human BECN1 / Beclin-1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • ICC
  • Western blot (0.5 - 1 µg/ml)
  • ELISA

Specificity and Use

BECN1 / Beclin-1 antibody was raised against synthetic peptide from human BECN1 / Beclin-1.
LS-E30177 - Liquid - 50 µg - $145.00
C-Terminus
16 amino acid peptide from near the carboxy terminus of human Beclin-1
Immunohistochemistry: LS-B3202 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3202 was determined to be 2.5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About BECN1 / Beclin-1

Q14457 NM_003766 NP_003757.1

BECN1 Antibody, ATG6 Antibody, Beclin 1 Antibody, Beclin 1, autophagy related Antibody, Beclin1 Antibody, GT197 Antibody, Protein GT197 Antibody, VPS30 Antibody, Beclin-1 Antibody

Beclin 1 is a coiled coil protein that interacts with prototypic cellular antiapoptotic gene Bcl2. Beclin 1 is widely expressed with highest levels reported in skeletal muscle. Three alternativaly spliced isoforms have been identified. Allelic deletion of beclin 1 results in a wide variety of cancers including breast cancer.

Immunohistochemistry

Immunohistochemistry
Anti-BECN1 / Beclin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3202 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-BECN1 / Beclin-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3202 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-BECN1 / Beclin-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3202 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry staining of A431 cells using Beclin-1 antibody at 1 µg/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of Beclin-1 in A431 cells with Beclin-1 antibody at 2 ug/ml.

Western blot

Western blot
Western blot of Beclin-1 in A431 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

Requested From: United States
Date Requested: 12/9/2016

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