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Anti-BDNF Antibody LS-C154099

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BDNF Antibodies

Anti-BDNF Antibody LS-C104728
Rabbit Polyclonal to Human BDNF
Human, Mouse, Rat
IHC, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-BDNF Antibody (aa183-194) IHC-plus™ LS-B3815
Goat Polyclonal to Human BDNF
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human BDNF
Human
Western blot, ELISA
Unconjugated

Details

Human BDNF
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • Western blot (1:1000)
  • ELISA

Specificity and Use

BDNF antibody was raised against this IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated human BDNF protein produced in E. coli.
This antibody is specific for human BDNF protein. A BLAST analysis was used to suggest cross-reactivity with BDNF from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to BDNF from a wide variety of animals. Cross-reactivity with BDNF from other sources has not been determined.
This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kD in size corresponding to the mature human BDNF protein by western blotting in appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About BDNF

P23560 NM_001709 NP_001700.2

BDNF Antibody, ANON2 Antibody, Abrineurin Antibody, BULN2 Antibody, Neurotrophin Antibody

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.

Western blot

Western blot
Anti-Human BDNF Antibody - Western Blot. Anti-human BDNF antibody in western blot shows detection of recombinant human BDNF raised in E. coli. Recombinant truncated (0.1 ug, 27 kD) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Human BDNF. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
 

Requested From: United States
Date Requested: 12/11/2016

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