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Anti-BDNF Antibody (Biotin) LS-C104729

Note: This antibody replaces LS-C53982, LS-C35182, LS-C187863

Ordering

Wt. Vol. Conc. Price
25 µg - - Unavailable
50 µg - - Unavailable

Related Products

129 BDNF Antibodies

Most Popular BDNF Antibodies

Anti-BDNF Antibody LS-C35180
Mouse Monoclonal (IgG1) to Human BDNF
Human
Western blot, ELISA, Neutralization
Unconjugated
Anti-BDNF Antibody LS-C77177
Mouse Monoclonal (IgG1) to Human BDNF
Human
Western blot, ELISA
Unconjugated
Anti-BDNF Antibody LS-C104728
Rabbit Polyclonal to Human BDNF
Human, Mouse, Rat
IHC, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-BDNF Antibody (aa183-194) IHC-plus™ LS-B3815
Goat Polyclonal to Human BDNF
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-BDNF Antibody IHC-plus™ LS-B11849
Chicken Polyclonal (IgY) to Mouse BDNF
Mouse, Human, Rat
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal to Human BDNF
Human, Mouse, Rat
IHC, Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Biotin Conjugated

Details

Human BDNF
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Biotin. Also available Unconjugated.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)
  • Neutralization
  • (applications tested for the base form of this product only)

Specificity and Use

BDNF antibody was raised against e.coli derived recombinant Human BDNF.
Human BDNF
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hBDNF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. To detect hBDNF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human BDNF (LS-C104728) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About BDNF

P23560 NM_001709 NP_001700.2

BDNF Antibody, ANON2 Antibody, Abrineurin Antibody, BULN2 Antibody, Neurotrophin Antibody

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.

Immunohistochemistry

Immunohistochemistry
IHC of BDNF antibody LS-C104728. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/24/2017

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