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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BCL2L14 / BCL‑G Antibody (C‑Terminus) IHC‑plus™ LS‑B1338
Note: This antibody replaces LS-C336
BCL-G antibody LS-B1338 is an unconjugated rabbit polyclonal antibody to BCL-G (BCL2L14) from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1338-50
50 µg (1 mg/ml)
Unavailable

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Product Description

BCL-G antibody LS-B1338 is an unconjugated rabbit polyclonal antibody to BCL-G (BCL2L14) from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About BCL2L14 / BCL-G
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. Q9BZR8 NM_030766 NP_110393.1

BCL2L14 Antibody, Bcl2-L-14 Antibody, BCLG Antibody, BCL-G Antibody, Apoptosis regulator Bcl-G Antibody


Specifications

Target
Human BCL2L14 / BCL-G
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (2.5 - 5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30028 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B1338. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Although antibody should react with both isoforms, only the Bcl-GS protein has been observed
Usage
Immunohistochemistry: LS-B1338 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1338 was determined to be 2 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-BCL2L14 / Bcl G antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.
Anti-BCL2L14 / Bcl G antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.

Immunohistochemistry

Anti-BCL2L14 / Bcl G antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.
Anti-BCL2L14 / Bcl G antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.

Immunofluorescence

Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 µg/mL.
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 µg/mL.

Immunofluorescence

Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 ug/ml.
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 ug/ml.

Western blot

Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 µg/mL.
Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 µg/mL.

Western blot

Western blot of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 ug/ml.
Western blot of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 ug/ml.

Immunohistochemistry

Anti-BCL2L14 / Bcl G antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.
Anti-BCL2L14 / Bcl G antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.

Immunohistochemistry

Anti-BCL2L14 / Bcl G antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.
Anti-BCL2L14 / Bcl G antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.

Immunofluorescence

Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 µg/mL.
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 µg/mL.

Immunofluorescence

Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 ug/ml.
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 ug/ml.

Western blot

Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 µg/mL.
Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 µg/mL.

Western blot

Western blot of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 ug/ml.
Western blot of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 ug/ml.

Immunohistochemistry

Anti-BCL2L14 / Bcl G antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.
Anti-BCL2L14 / Bcl G antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.

Immunohistochemistry

Anti-BCL2L14 / Bcl G antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.
Anti-BCL2L14 / Bcl G antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.

Immunofluorescence

Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 µg/mL.
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 µg/mL.

Immunofluorescence

Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 ug/ml.
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 ug/ml.

Western blot

Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 µg/mL.
Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 µg/mL.

Western blot

Western blot of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 ug/ml.
Western blot of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 ug/ml.

Immunohistochemistry

Anti-BCL2L14 / Bcl G antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.
Anti-BCL2L14 / Bcl G antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.

Immunohistochemistry

Anti-BCL2L14 / Bcl G antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.
Anti-BCL2L14 / Bcl G antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1338 concentration 2 ug/ml.

Immunofluorescence

Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 µg/mL.
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 µg/mL.

Immunofluorescence

Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 ug/ml.
Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 ug/ml.

Western blot

Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 µg/mL.
Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 µg/mL.

Western blot

Western blot of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 ug/ml.
Western blot of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 ug/ml.


Requested From: 
Date Requested: 6/18/2018

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