Human BCL2L1 / BCL-XL

Mouse

Human, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

2H12

Unconjugated

Protein G purified

Ready-to-Use. Also available Unmodified or Azide-free, BSA-free.

Synthetic peptide (QSNRELVVDFLS, aa3-14 of UniProt Q07817) from the human protein. Percent identity by BLAST analysis: Human, Mouse, Rat, Ferret, Sheep, Cat, Bovine, Bat, Hamster, Elephant, Rabbit, Horse, Pig, Dog (100%); Guinea pig (92%); Opossum, Lizard (83%).

aa3-14

Human BCL2L1 / Bcl-xL

• IHC
• IHC - Paraffin (1:1)
• Immunofluorescence
• Western blot
• Flow Cytometry

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins. The optimal dilution of the Bcl-X antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q07817 NCBI: NM_001191 NP_001182.1