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Anti-BCL2 / Bcl-2 Antibody (Ser70) IHC-plus™ LS-B1174

Note: This antibody replaces LS-C34296, LS-C74701, LS-C74704, LS-C118062, LS-C51196
Catalog Size Price
LS-B1174-50 50 µg (1 mg/ml) Unavailable

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Anti-BCL2 / Bcl-2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2352 concentration 5 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
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Formalin-fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and DAB chromogen. Note absence of staining of reactive cells in the germinal center.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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100% Guaranteed
Rabbit Polyclonal (IgG) to Human BCL2 / Bcl-2
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human BCL2 / Bcl-2
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot

Specificity and Use

BCL2 / Bcl-2 antibody was raised against synthetic peptide from human BCL2 / Bcl-2.
Ser70
Amino acids surrounding Serine 70 of human BCL2
Immunohistochemistry: LS-B1174 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1174 was determined to be 10 ug/ml.

Packaging

PBS, 0.02% sodium azide, 50% glycerol.
4°C or -20°C, Avoid freeze-thaw cycles.
For research use only.

About BCL2 / Bcl-2

P10415 NM_000633 NP_000624.2

BCL2 Antibody, Apoptosis regulator Bcl-2 Antibody, B-cell CLL/lymphoma 2 Antibody, B-cell lymphoma protein 2 Antibody, Bcl-2 Antibody, PPP1R50 Antibody

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Immunohistochemistry

Anti-BCL2 / Bcl-2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1174 concentration 10 ug/ml.
Anti-BCL2 / Bcl-2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1174 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2(Ab-70) antibody.
Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2(Ab-70) antibody.

Western blot

Western blot of extracts from MCF cells using BCL-2(Ab-70) antibody and the same antibody preincubated with blocking peptide.
Western blot of extracts from MCF cells using BCL-2(Ab-70) antibody and the same antibody preincubated with blocking peptide.

Requested From: 
Date Requested: 5/24/2018

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