Human BCL2 / Bcl-2

Mouse

Human, Chimpanzee, Monkey, Dog, Pig (tested or 100% immunogen sequence identity)

Mouse, Rat

IgG1,k Monoclonal

8C8

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified or Ready-to-Use.

A synthetic peptide, aa 41-54 (G aa PAPGIFSSQPG-Cys) of human Bcl-2 protein. Percent identity by BLAST analysis: Human, Chimpanzee, Cat, Pig, Dog (100%); Gorilla, Orangutan, Bovine, Goat (91%); Galago (82%).

aa41-54

Human BCL2 / Bcl-2

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Immunoprecipitation
• Flow Cytometry (0.5 - 1 µg/10E6 cells)
• ELISA

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative. ELISA: Order BSA-free format for coating.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10415 NCBI: NM_000633 NP_000624.2