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Anti-BCL10 / BCL-10 Antibody (clone 4A8) LS-C115256

Catalog Size Price
LS-C115256-100 100 µl Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 4A8] (IgG1) to Human BCL10 / BCL-10
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human BCL10 / BCL-10
Mouse
IgG1 Monoclonal [4A8]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

BCL10 / BCL-10 antibody was raised against full length human recombinant protein of human BCL10 (NP_003912) produced in HEK293T cell
Human BCL10
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About BCL10 / BCL-10

O95999 NM_003921 NP_003912.1

BCL10 Antibody, Bcl-10 Antibody, B-cell CLL/lymphoma 10 Antibody, B-cell lymphoma/leukemia 10 Antibody, CARMEN Antibody, CARD-like apoptotic protein Antibody, CCARMEN Antibody, C-E10 Antibody, CIPER Antibody, Cellular-E10 Antibody, HCLAP Antibody, Cellular homolog of vCARMEN Antibody, CLAP Antibody, ME10 Antibody

Apoptosis is related to many diseases including cancer. Cell death signals are transduced by death domain (DD) and caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include ARC, RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD containing protein was recently identified by several groups and designated Bcl10, CIPER, mE10, CARMEN, CLAP. Bcl10 is a cellular homolog of the equine herpesvirus-2 E-10 gene.

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-BCL10 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-BCL10 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-BCL10 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-BCL10 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BCL10 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BCL10 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-BCL10 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCL10.
Anti-BCL10 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCL10.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL10.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL10.

Flow Cytometry

Flow cytometric Analysis of Jurkat cells, using anti-BCL10 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-BCL10 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Requested From: 
Date Requested: 5/21/2018

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