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Catalog Number Size Price
LS-C353193-100 100 µl (1 mg/ml) $315 
BCAR1 / p130Cas Antibody - Immunofluorescent analysis of p130 Cas (pY249) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
BCAR1 / p130Cas Antibody - Western blot analysis of p130 Cas (pY249) expression in HEK293T EGF-treated (A); NIH3T3 EGF-treated (B); Raw264.7 EGF-treated (C); PC12 EGF-treated (D) whole cell lysates.
BCAR1 / p130Cas Antibody - Immunofluorescent analysis of p130 Cas (pY249) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
BCAR1 / p130Cas Antibody - Western blot analysis of p130 Cas (pY249) expression in HEK293T EGF-treated (A); NIH3T3 EGF-treated (B); Raw264.7 EGF-treated (C); PC12 EGF-treated (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human BCAR1 / p130Cas Antibody (phospho‑Tyr249, IF, WB) LS‑C353193

Polyclonal Rabbit anti‑Human BCAR1 / p130Cas Antibody (phospho‑Tyr249, IF, WB) LS‑C353193

Antibody:
BCAR1 / p130Cas Rabbit anti-Human Polyclonal (pTyr249) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$315
LS-C353193-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BCAR1 / p130Cas Rabbit anti-Human Polyclonal (pTyr249) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
P130Cas antibody LS-C353193 is an unconjugated rabbit polyclonal antibody to p130Cas (BCAR1) (pTyr249) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and WB.
Target
Human BCAR1 / p130Cas
Synonyms
BCAR1 | CAS | CAS1 | CRKAS | CASS1 | CRK-associated substrate | p130Cas
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human p130 Cas.
Epitope
pTyr249
Specificity
Recognizes endogenous levels of p130 Cas (pY249) protein.
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BCAR1 / p130Cas
P56945 NM_014567 NP_055382.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024