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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BCAM / CD239 Antibody (aa459‑508) IHC‑plus™ LS‑B11547
Note: This antibody replaces LS-C135747
CD239 antibody LS-B11547 is an unconjugated rabbit polyclonal antibody to CD239 (BCAM) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B11547-50
50 µg
$485

Popular BCAM / CD239 Products

Antibody
Species: Human
Applications: Western blot, Flow Cytometry
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Mouse, Rat, Bovine, Dog, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Immunohistochemistry of Human prostate cancer using BCAM Polyclonal Antibody at dilution of 1:40.
Species: Human, Mouse
Applications: IHC, Western blot, ELISA

Product Description

BCAM / CD239 Antibody (aa459-508) for IHC, WB/Western IHC-plus™ LS-B11547

Specifications

Target
Human BCAM / CD239
Synonyms
BCAM, B-cell adhesion molecule, Auberger B antigen, Basal cell adhesion molecule, CD239 antigen, Glycoprotein 95kDa, F8/G253 antigen, LU, Lutheran antigen, MSK19, AU, CD239
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BCAM / CD239 antibody was raised against synthetic peptide located between aa459-508 of human BCAM (P50895, NP_005572). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Monkey, Marmoset, Dog, Rabbit (85%); Horse (84%); Mouse, Rat (78%).
Blocking Peptide
LS-E22049 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B11547. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa459-508
Specificity
Human BCAM
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BCAM / CD239
BCAM / CD239 is Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. P50895 NM_005581 NP_005572.2


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Images

Immunohistochemistry

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

BCAM antibody western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
BCAM antibody western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

BCAM antibody western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
BCAM antibody western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

BCAM antibody western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
BCAM antibody western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

BCAM antibody western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
BCAM antibody western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/21/2018

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