Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin
Specificity and Use
BCA225 (BRST-1) antibody was raised against protein secreted by the T47D (Clone 11) human breast carcinoma cell line.
Recognizes human BCA-225.
Immunohistochemistry (frozen sections, paraffin sections). Identifies BCA-225, a 225-250kD glycoprotein which produces strong intracellular staining of primary and metastatic breast carcinoma tissue as well as in cervical carcinomas. Apical/glycocalyx staining is seen in normal kidney, lung, fallopian tube, liver, skin (eccrine sweat glands) and uterus. Similar staining patterns are observed in lung, ovarian and endometrial cancers. Carcinomas of the colon, stomach, prostate, urinary bladder, liver, pancreas, thyroid and parotid are negative, as are sarcomas and lymphoid cancers.
PBS, pH 7.4, 0.1% sodium azide, 1% BSA.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.