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Anti-BBC3 / PUMA Antibody (aa2-16) IHC-plus™ LS-B7285

Note: This antibody replaces LS-C42938, LS-C124463

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BBC3 / PUMA Antibodies

Anti-BBC3 / PUMA Antibody (C-Terminus) IHC-plus™ LS-B1334
Rabbit Polyclonal to Human BBC3 / PUMA
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human BBC3 / PUMA
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (2 - 4 µg/ml)

Specificity and Use

BBC3 / PUMA antibody was raised against synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human PUMA alpha. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%); Opossum (87%).
aa2-16
Recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kD pro-apoptotic member of the BCL-2 family. Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53. Four isoforms of PUMA have been described due to alternative splicing. Is expected to recognize the canonical isofom 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA.
Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Histology Positive Control Tissue: Human breast carcinoma. Western Blot: detects a band of approximately 23kD in K562 cell lysates.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About BBC3 / PUMA

Q9BXH1 NM_014417 NP_055232.1

BBC3 Antibody, Bcl-2-binding component 3 Antibody, BCL2 binding component 3 Antibody, JFY-1 Antibody, JFY1 Antibody, PUMA Antibody

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53.

Immunohistochemistry

Immunohistochemistry
Anti-BBC3 / PUMA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7285 concentration 2.5 ug/ml.
 

Requested From: United States
Date Requested: 12/10/2016

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