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Anti-BBC3 / PUMA Antibody (aa119-154) LS-C98860

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BBC3 / PUMA Antibodies

Anti-BBC3 / PUMA Antibody (C-Terminus) IHC-plus™ LS-B1334
Rabbit Polyclonal to Human BBC3 / PUMA
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human BBC3 / PUMA
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human BBC3 / PUMA
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

BBC3 / PUMA antibody was raised against kLH-conjugated synthetic peptide between amino acids 119-154 from human Puma BH3 Domain.
LS-E2249 - Lyophilized - 100 µg - $145.00
aa119-154

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About BBC3 / PUMA

Q9BXH1 NM_014417 NP_055232.1

BBC3 Antibody, Bcl-2-binding component 3 Antibody, BCL2 binding component 3 Antibody, JFY-1 Antibody, JFY1 Antibody, PUMA Antibody

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of Puma BH3 Domain Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
Western blot of anti-Puma BH3 domain antibody in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Flow Cytometry

Flow Cytometry
Puma BH3 Domain Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 12/8/2016

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