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Catalog Number Size Price
LS-C98860-400 400 µl $393 
BBC3 / PUMA Antibody - Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
BBC3 / PUMA Antibody - Confocal immunofluorescence of Puma BH3 Domain Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
BBC3 / PUMA Antibody - Western blot of anti-Puma BH3 domain antibody in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
BBC3 / PUMA Antibody - Puma BH3 Domain Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
BBC3 / PUMA Antibody - Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
BBC3 / PUMA Antibody - Confocal immunofluorescence of Puma BH3 Domain Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
BBC3 / PUMA Antibody - Western blot of anti-Puma BH3 domain antibody in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
BBC3 / PUMA Antibody - Puma BH3 Domain Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human BBC3 / PUMA Antibody (aa119‑154, IHC, IF, WB) LS‑C98860

Polyclonal Rabbit anti‑Human BBC3 / PUMA Antibody (aa119‑154, IHC, IF, WB) LS‑C98860

Antibody:
BBC3 / PUMA Rabbit anti-Human Polyclonal (aa119-154) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C98860-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BBC3 / PUMA Rabbit anti-Human Polyclonal (aa119-154) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PUMA antibody LS-C98860 is an unconjugated rabbit polyclonal antibody to PUMA (BBC3) (aa119-154) from human. It is reactive with human and mouse. Validated for Flow, IF, IHC and WB. Cited in 1 publication.
Target
Human BBC3 / PUMA
Synonyms
BBC3 | Bcl-2-binding component 3 | BCL2 binding component 3 | JFY-1 | JFY1 | PUMA | PUMA alpha
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa119-154
Specificity
This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BBC3 / PUMA
Q9BXH1 NM_014417 NP_055232.1

LSBio Ratings

BBC3 / PUMA Antibody (aa119-154) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-C98860 has an LSBio Rating of
Publications (4)

Publications (1)

Bcl-2 Is a Therapeutic Target for Hypodiploid B-Lineage Acute Lymphoblastic Leukemia. Ernesto Diaz-Flores, Evan Q Comeaux, Kailyn L Kim, Ella Melnik, Kyle Beckman, Kara L Davis, Kevin Wu, Jon Akutagawa, Olga Bridges, Roberta Marino, Margo Wohlfeil, Benjamin S Braun, Charles G Mullighan, Mignon L Loh. Cancer research. 2019 May;79:2339-2351.

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/19/2024