Target
Human BAX
Synonyms
BAX | BCL2-associated X protein | Bcl2-L-4 | Apoptosis regulator BAX | Bcl-2-like protein 4 | BCL2L4
Reactivity
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide (KLH coupled) corresponding to the N-Terminus residues of human Bax.
Specificity
Detects endogenous levels of total Bax protein independent of phosphorylation. The antibody does not cross-react with other Bcl-2 family members. Species cross-reactivity: Human, monkey, mouse and rat.
Applications
- Western blot (1:1000)
- Immunoprecipitation (1:50)
- ELISA
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Immunoprecipitation: 1:50. Western Blot: 1:1000. Note: incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee