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Catalog Number Size Price
LS-C351882-100 100 µl (1 mg/ml) $299 
BAX Antibody - Immunohistochemical analysis of BAX staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
BAX Antibody - Immunofluorescent analysis of BAX staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
BAX Antibody - Western blot analysis of BAX expression in HeLa (A); A549 (B); A375 (C); Jurkat (D); HepG2 (E); PC12 (F) whole cell lysates.
BAX Antibody - Immunohistochemical analysis of BAX staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
BAX Antibody - Immunofluorescent analysis of BAX staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
BAX Antibody - Western blot analysis of BAX expression in HeLa (A); A549 (B); A375 (C); Jurkat (D); HepG2 (E); PC12 (F) whole cell lysates.
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Polyclonal Rabbit anti‑Human BAX Antibody (Internal, IHC, IF, WB) LS‑C351882

Polyclonal Rabbit anti‑Human BAX Antibody (Internal, IHC, IF, WB) LS‑C351882

Antibody:
BAX Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C351882-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BAX Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
BAX antibody LS-C351882 is an unconjugated rabbit polyclonal antibody to BAX (Internal) from human. It is reactive with human, rat, bovine and other species. Validated for ELISA, ICC, IF, IHC and WB.
Target
Human BAX
Synonyms
BAX | BCL2-associated X protein | Bcl2-L-4 | Apoptosis regulator BAX | Bcl-2-like protein 4 | BCL2L4
Host
Rabbit
Reactivity
Human, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human BAX.
Epitope
Internal
Specificity
Recognizes endogenous levels of BAX protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • ELISA (1:5000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAX
Q07812 NM_004324 NP_004315.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/24/2024