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Anti-BAX Antibody (clone BAX/962, Azide-free) LS-C390893

Catalog Size Price
LS-C390893-100 100 µg (1 mg/ml) Unavailable

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Anti-BAX Antibody (aa1-50) IHC-plus™ LS-B6546
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100% Guaranteed
Mouse Monoclonal [clone BAX/962] (IgG1) to Human BAX
Human, Monkey
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry (applications tested for the base form of this product only)
Unconjugated, Azide-free

Details

Human BAX
Mouse
Human, Monkey (tested or 100% immunogen sequence identity)
Mouse, Rat
IgG1 Monoclonal [BAX/962]
Unconjugated
Protein G purified
Azide-free. Also available Unmodified or Ready-to-Use.

Applications

  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)

Specificity and Use

BAX antibody was raised against recombinant Full-length Human Protein.
Human BAX
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Optimal dilution of the Bax antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

Packaging

Sterile PBS.
2°C to 8°C
For research use only.

About BAX

Q07812 NM_004324 NP_004315.1

BAX Antibody, BCL2-associated X protein Antibody, Bcl2-L-4 Antibody, Apoptosis regulator BAX Antibody, Bcl-2-like protein 4 Antibody, BCL2L4 Antibody

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed, paraffin-embedded human melanoma stained with Bax antibody (BAX/962). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed, paraffin-embedded Hodgkin's lymphoma stained with Bax antibody (BAX/962). This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 10/18/2017

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