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Anti-BAX Antibody (clone 6A7) IHC-plus™ LS-B2510

Note: This antibody replaces LS-C41063

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular BAX Antibodies

Anti-BAX Antibody (clone 6A7) IHC-plus™ LS-B2510
Mouse Monoclonal [clone 6A7] (IgG1) to Human BAX
Human, Monkey, Mouse, Rat
IHC - Paraffin, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-BAX Antibody (N-Terminus) LS-C127000
Rabbit Polyclonal (IgG) to Human BAX
Human, Mouse, Rat, Rabbit
Western blot
Unconjugated
Anti-BAX Antibody (aa43-60) LS-C148225
Rabbit Polyclonal to Mouse BAX
Mouse, Rat
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 6A7] (IgG1) to Human BAX
Human, Monkey, Mouse, Rat
IHC - Paraffin, Immunoprecipitation
Unconjugated

Details

Human BAX
Mouse
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [6A7]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (10 - 12 µg/ml)
  • Immunoprecipitation

Specificity and Use

BAX antibody was raised against synthetic peptide from human BAX.
Amino acid:12-24 common to human, mouse, and rat Bax protein.
Immunohistochemistry: LS-B2510 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2510 was determined to be 10 ug/ml.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml.
+4°C. Store undiluted.
For research use only.

About BAX

Q07812 NM_004324 NP_004315.1

BAX Antibody, BCL2-associated X protein Antibody, Bcl2-L-4 Antibody, Apoptosis regulator BAX Antibody, Bcl-2-like protein 4 Antibody, BCL2L4 Antibody

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death.

Immunohistochemistry

Immunohistochemistry
Anti-BAX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2510 concentration 10 ug/ml.

Immunoprecipitation

Immunoprecipitation
Extracts from Hela cell lysate was immunoprecipitated with clone 6A7 (lane 1), 4 ug purified monoclonal antibody without cell lysate (clone 6A7, lane 2), or Hela cell lysate without immunoprecipitation (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Bax polyclonal antibody.

Requested From: United States
Date Requested: 12/4/2016

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