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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Basigin / Emmprin / CD147 Antibody (clone 4D3) LS‑C115266
CD147 antibody LS-C115266 is an unconjugated mouse monoclonal antibody to human CD147 (Basigin / Emmprin). Validated for IF, IHC and WB.
Catalog
Size
Price
LS-C115266-100
100 µl
Unavailable

Popular Basigin / Emmprin / CD147 Products

Anti-CD147 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1861 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Anti-CD147 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1950 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Anti-CD147 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3167 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Anti-CD147 antibody IHC of mouse testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3663 concentration 10 ug/ml.
Species: Mouse
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Anti-CD147 antibody IHC of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4883 concentration 10 ug/ml.
Species: Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry

Product Description

CD147 antibody LS-C115266 is an unconjugated mouse monoclonal antibody to human CD147 (Basigin / Emmprin). Validated for IF, IHC and WB.
About Basigin / Emmprin / CD147
Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. P35613 NM_001728 NP_001719.2

BSG Antibody, 5F7 Antibody, Basigin Antibody, Basigin (Ok blood group) Antibody, CD147 antigen Antibody, Collagenase stimulatory factor Antibody, EMMPRIN Antibody, OK Antibody, OK blood group antigen Antibody, TCSF Antibody, CD147 Antibody, M6 Antibody


Specifications

Target
Human Basigin / Emmprin / CD147
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [4D3]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human BSG(NP_001719) produced in HEK293T cell.
Specificity
Human Emmprin / CD147
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human liver tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human prostate tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-BSG mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG.
Anti-BSG mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-BSG monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-BSG monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human liver tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human prostate tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-BSG mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG.
Anti-BSG mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-BSG monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-BSG monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human liver tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human prostate tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-BSG mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG.
Anti-BSG mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-BSG monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-BSG monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human liver tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human prostate tissue using anti-BSG mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-BSG mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG.
Anti-BSG mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-BSG monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-BSG monoclonal antibody.


Requested From: 
Date Requested: 6/21/2018

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