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Anti-Basic Cytokeratin AE3 Antibody (clone AE3) IHC-plus™ LS-B5557

Note: This antibody replaces LS-C134824

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Basic Cytokeratin AE3 Antibodies

Anti-Basic Cytokeratin AE3 Antibody (clone AE3) LS-C71285
Mouse Monoclonal [clone AE3] (IgG) to Human Basic Cytokeratin AE3
Human, Mouse, Rat, Rabbit
IHC - Paraffin, IHC - Frozen
Unconjugated
Anti-Basic Cytokeratin AE3 Antibody (clone AE3) IHC-plus™ LS-B5557
Mouse Monoclonal [clone AE3] (IgG1) to Human Basic Cytokeratin AE3
Human
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Immunohistochemistry Image
Anti-Basic Cytokeratin AE3 Antibody (clone AE3) IHC-plus™ LS-B8611
Mouse Monoclonal [clone AE3] (IgG1) to Human Basic Cytokeratin AE3
Human, Monkey, Mouse, Rat, Bovine, Rabbit, Chicken
IHC - Paraffin, Immunofluorescence
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone AE3] (IgG1) to Human Basic Cytokeratin AE3
Human
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated

Details

Human Basic Cytokeratin AE3
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [AE3]
Unconjugated
Purified IgG
Unmodified

Applications

  • IHC - Paraffin (1:100)
  • IHC - Frozen
  • Western blot (1:100)

Specificity and Use

Human epidermal cells.
This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is broadly reactive with almost all epithelial and there neoplasms. This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. The antibody is cross-reactive with keratins of rabbit, mouse, rat, bovine and chicken. Within immunoblots AE-1 detects distinct MW's of 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip's lower then 6.0. These MW's are believed to correspond with respectively keratin 8, 7, 6, 5, 4, 3 and 1 & 2.
In unfixed frozen sections and formalin-fixed paraffin embedded tissues. Also applicable in Western blotting. For use on formalin-fixed paraffin embedded tissues a dilution of approximately 1:150 to 1:200 is appropriate if section are incubated for 30 min. at 37°C. Possibly, higher dilutions can be used if sections are incubated at 4°C overnight. For use in Western blotting a dilution of 1:100 is advised.

Packaging

0.01M PBS containing 0.1% Sodium azide and 1% BSA. Sourced in Ascites.
Store at 4°C.
For research use only.

About Basic Cytokeratin AE3

The monoclonal antibody AE3 recognizes many members of the basic (Type II) cytokeratin family. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. The AE3 monoclonal antibody recognizes the 65 to 67 triplet, 64, 59, 58, 56, 54 and 52kD proteins (also known as cytokeratin 1, 2, 3, 4, 5, 6, 7 and 8) in mouse, human, rat, primate (cynomolgus and rhesus), dog, cat, rabbit and chicken.

Immunohistochemistry

Immunohistochemistry
Anti-Cytokeratin Type II antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5557 dilution 1:100.

Immunohistochemistry

Immunohistochemistry
Anti-Cytokeratin Type II antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5557 dilution 1:100.

Requested From: United States
Date Requested: 12/4/2016

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