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Anti-BAR / BFAR Antibody (aa3-21) LS-C148222

Ordering

Wt. Vol. Conc. Price
- 50 µl - $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BAR / BFAR Antibodies

Anti-BAR / BFAR Antibody (C-Terminus) IHC-plus™ LS-B4629
Rabbit Polyclonal to Human BAR / BFAR
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-BAR / BFAR Antibody (aa1-30, APC) LS-C233142
Rabbit Polyclonal (IgG) to Human BAR / BFAR
Human
Western blot, ELISA
APC Conjugated
Anti-BAR / BFAR Antibody (aa1-30, Biotin) LS-C233143
Rabbit Polyclonal (IgG) to Human BAR / BFAR
Human
Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human BAR / BFAR
Human
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated

Details

Human BAR / BFAR
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC - Paraffin (1:1000 - 1:5000)
  • IHC - Frozen
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)

Specificity and Use

BAR / BFAR antibody was raised against a synthetic peptide corresponding to amino acids 3-21 (EPQKSYVNTMDLERDEPLK) of human BAR, GenBank no. NP_057645.1. Human BAR is a 450 amino acid protein. Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (84%).
aa3-21
BAR.

Packaging

Antiserum, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About BAR / BFAR

Q9NZS9 NM_016561 NP_057645.1

BFAR Antibody, BAR Antibody, RNF47 Antibody, RING finger protein 47 Antibody

Apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed, paraffin-embedded tissue sections stained for BAR expression using LS-C148222 at 1:2000. A. Two cores from a human glioblastoma tissue microarray: 1 =fibrillary astrocytoma (grade I), and2 = anaplastic glioma (grade III). B. Higher magnification from the fibrillary astrocytoma (shown in A). C. Higher magnification from the anaplastic glioma (shown in A). D. Normal human brain striatum with positive medium spiny neurons, the major neuronal cell type of the striatum. Hematoxylin-Eos in counterstain.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue array stained for BAR expression using LS-C148222 at 1:2000. Hematoxylin-Eosin counterstain. Variable BAR expression is seen between patient samples.

Western blot

Western blot
Western blot of BAR in normal prostate and prostate carcinoma tissue lysates. 25 ug protein was loaded per lane. Tissue lysates from 15 different prostate carcinoma patients show variable expression of BAR with respect to banding patterns and amount of BAR expression. Major BAR bands typically migrate as a single band or as a doublet. These bands are typically observed at ~50-54 kD. This is higher than the predicted molecular weight from the 450 amino acid BAR sequence, and may represent phosphorylation or other post-translational modifications. N = tissue lysate from normal prostate.
 

Requested From: United States
Date Requested: 12/4/2016

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