Human BAG4 / SODD

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-GNATSDHPNNQDQSS, from the internal region of the protein sequence according to NP_004865.1.

aa349-363

Human BAG4 / SODD.

• IHC
• IHC - Paraffin (3.75 µg/ml)
• Western blot (0.3 - 1 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:8000)

Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx. 60kDa band observed in cell lines HEK293 and HeLa lysates (calculated MW of 49.6kDa according to NP_004865.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Takada et al, Mol Cell Biol. 2003 Jun;23(11):4026-33.; PMID: 12748303). Recommended concentration: 0.3-1 ug/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O95429 NCBI: NM_004874 NP_004865.1