Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Human BAG3 / BAG-3
Human, Monkey (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:1000 - 1:5000)
IHC - Frozen
Western blot (1:1000 - 1:2000)
Immunoprecipitation (1:50 - 1:200)
Specificity and Use
BAG3 / BAG-3 antibody was raised against a synthetic peptide corresponding to amino acids 276-294 (REGSPARSSTPLHSPSPIR) of human BAG-3, GenBank no. gi|14043024|ref|NP_004272.2| The amino acid sequence used for immunogen is 84% conserved with rat and mouse BAG-3. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%); Gorilla (95%); Hamster, Elephant, Horse (89%); Mouse, Rat, Panda, Rabbit (84%).
Antiserum, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
BAG3 Antibody, Bcl-2-associated athanogene 3 Antibody, Bcl-2-binding protein Bis Antibody, BCL2-binding athanogene 3 Antibody, BIS Antibody, BAG-3 Antibody, CAIR-1 Antibody, Docking protein cair1 Antibody, BCL2-associated athanogene 3 Antibody, Docking protein CAIR-1 Antibody
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells.
Western blot of BAG-3 in CAPAN-1 human pancreatic cancer cells following heat shock using LS-C148217 at !:2000, Cells were heat shocked at 45 degrees for 10min and total proteins were extracted following incubation at 37 degrees for the indicated times. Control= no heat shock treatment. Maxi mal BAG-3 protein expression was detected at 6 h post heat shock treatment.
Western blot of BAG-3 in human pancreatic cancer cell lines using LS-C148217 at 1:2000.
Requested From: United States
Date Requested: 1/19/2017