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Anti-BAG3 / BAG-3 Antibody (aa276-294) LS-C148217


Wt. Vol. Conc. Price
- 50 µl - Unavailable

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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human BAG3 / BAG-3
Human, Monkey
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation


Human BAG3 / BAG-3
Human, Monkey (tested or 100% immunogen sequence identity)


  • IHC - Paraffin (1:1000 - 1:5000)
  • IHC - Frozen
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)

Specificity and Use

BAG3 / BAG-3 antibody was raised against a synthetic peptide corresponding to amino acids 276-294 (REGSPARSSTPLHSPSPIR) of human BAG-3, GenBank no. gi|14043024|ref|NP_004272.2| The amino acid sequence used for immunogen is 84% conserved with rat and mouse BAG-3. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%); Gorilla (95%); Hamster, Elephant, Horse (89%); Mouse, Rat, Panda, Rabbit (84%).
Human BAG3.


Antiserum, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About BAG3 / BAG-3

O95817 NM_004281 NP_004272.2

BAG3 Antibody, Bcl-2-associated athanogene 3 Antibody, Bcl-2-binding protein Bis Antibody, BCL2-binding athanogene 3 Antibody, BIS Antibody, BAG-3 Antibody, CAIR-1 Antibody, Docking protein cair1 Antibody, BCL2-associated athanogene 3 Antibody, Docking protein CAIR-1 Antibody

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells.

Western blot

Western blot
Western blot of BAG-3 in CAPAN-1 human pancreatic cancer cells following heat shock using LS-C148217 at !:2000, Cells were heat shocked at 45 degrees for 10min and total proteins were extracted following incubation at 37 degrees for the indicated times. Control= no heat shock treatment. Maxi mal BAG-3 protein expression was detected at 6 h post heat shock treatment.

Western blot

Western blot
Western blot of BAG-3 in human pancreatic cancer cell lines using LS-C148217 at 1:2000.

Requested From: 
Date Requested: 3/23/2017

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