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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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BAF53 / ACTL6A Antibody (aa81‑130) LS‑C201682
ACTL6A antibody LS-C201682 is an unconjugated rabbit polyclonal antibody to ACTL6A (BAF53) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C201682-50
50 µg (1 mg/ml)
Unavailable

Popular BAF53 / ACTL6A Products

Human, Skin: Formalin-Fixed Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western Blot analysis of ACTL6A expression in transfected 293T cell line by ACTL6A monoclonal antibody (M10), clone 3C4.Lane 1: ACTL6A transfected lysate(47.5 KDa).Lane 2: Non-transfected lysate.
Species: Human
Applications: Western blot, ELISA
Western blot analysis of extracts of various cell lines.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, ChIP-sequencing
Immunohistochemical analysis of BAF53A staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Proximity Ligation Assay

Product Description

ACTL6A antibody LS-C201682 is an unconjugated rabbit polyclonal antibody to ACTL6A (BAF53) from human, mouse, rat and other species. Validated for WB.
About BAF53 / ACTL6A
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. O96019 NM_004301 NP_004292.1

ACTL6A Antibody, ACTL6 Antibody, Actin-like 6A Antibody, Actin-related protein 4 Antibody, Actin-related protein Baf53a Antibody, ArpNbeta Antibody, BAF53 Antibody, ARPN-BETA Antibody, BAF53A Antibody, BRG1-associated factor 53A Antibody, Actin-like protein 6A Antibody, INO80 complex subunit K Antibody, ARP4 Antibody, BAF complex 53 kDa subunit Antibody, HArpN beta Antibody, INO80K Antibody


Specifications

Target
Human BAF53 / ACTL6A
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Xenopus (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
BAF53 / ACTL6A antibody was raised against synthetic peptide located between aa81-130 of human ACTL6A (D3DNS0). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Lizard, Xenopus (100%); Hamster, Turkey, Zebra finch, Chicken, Salmon, Stickleback, Medaka, Pufferfish, Zebrafish (84%).
Epitope
aa81-130
Specificity
Human BAF53 / ACTL6A
Presentation
Lyophilized from PBS, 2% sucrose.
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of Human THP-1. ACTL6A antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human THP-1. ACTL6A antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human THP-1. ACTL6A antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human THP-1. ACTL6A antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human THP-1. ACTL6A antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human THP-1. ACTL6A antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human THP-1. ACTL6A antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human THP-1. ACTL6A antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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